|
| Status |
Public on Jul 01, 2014 |
| Title |
Stroke Paxgene blood VAL-50 |
| Sample type |
RNA |
| |
|
| Source name |
Paxgene venous blood
|
| Organism |
Homo sapiens |
| Characteristics |
diagnosis: Ischemic Stroke
tissue: venous blood
|
| Treatment protocol |
PAXgene tube, frozen -80'C
|
| Growth protocol |
Venous blood draw into PAXgene tube
|
| Extracted molecule |
total RNA |
| Extraction protocol |
PAXgene blood miRNA kit, Pre-AnalytiX, Quantity and quality of RNA was determined by Nano-Drop (Thermo Fisher) and Agilent 2100 Bioanalyzer (Agilent). Samples required A260/A280 absorbance ratio ≥1.8, A260/A230 ≥1.8, 28S/18S rRNA ratio ≥1.8, and an RNA integrity number ≥8.
|
| Label |
biotin
|
| Label protocol |
200ng total RNA labelled with FlashTag Biotin HSR labeling kits (Affymetrix)
|
| |
|
| Hybridization protocol |
Affymetrix miRNA GeneChip 3.0 protocol, 45'C 18hr GeneChip Hybridization Oven 645
|
| Scan protocol |
Affymetrix GCS3000 Gene Array Scanner
|
| Description |
miRNA expression from human blood cells
|
| Data processing |
Partek Genomics Suite 6.6, RMA normalization, log2 transformation, hsa-miR filter
|
| |
|
| Submission date |
Mar 14, 2014 |
| Last update date |
Jul 01, 2014 |
| Contact name |
Glen Jickling |
| E-mail(s) |
gcjickling@ucdavis.edu
|
| Organization name |
University of California Davis
|
| Street address |
2805 50th St
|
| City |
Sacramento |
| State/province |
CA |
| ZIP/Postal code |
95817 |
| Country |
USA |
| |
|
| Platform ID |
GPL16384 |
| Series (1) |
|
