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Sample GSM1350819 Query DataSets for GSM1350819
Status Public on Feb 19, 2019
Title Balb.Lb_2
Sample type RNA
 
Source name whole mouse ear with Leishmania lesion
Organism Mus musculus
Characteristics strain of mouse: Balb/C
species of leishmania: braziliensis
susceptibility phenotype: resistant
tissue: whole mouse ear
Treatment protocol Ear tissue was preserved in RNAlater reagent (Qiagen) and stored at -80c.
Growth protocol 6-8wk old mice were infected with 105 metacyclic-enriched parasites. Mice were killed at 4 weeks post-infection and the infected ear was recovered.
Extracted molecule total RNA
Extraction protocol RNAlater-preserved ear tissue was weighed, minced with a razor blade, and the entire ear was homogenized in 1ml of Qiazol (Qiagen) using 3 x 20 second pulses on a FastPrep-24 MP homogenizer (MP Biomedicals), with 5 min on ice between each pulse. Total RNA was isolated using the Qiagen miRNeasy kit.
Label biotin
Label protocol Biotin labeled complementary RNA (cRNA) was made using the EpiCentre Nano Total-Amp kit
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description 8762430076_E
Data processing The data were quantile normalized without background subtraction using Illumina GenomeStudio V1.8
 
Submission date Mar 19, 2014
Last update date Feb 19, 2019
Contact name daniel beiting
E-mail(s) beiting@vet.upenn.edu
Organization name University of Pennsylvania
Department Pathobiology
Lab Beiting
Street address 380 S. University Ave
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
 
Platform ID GPL6885
Series (1)
GSE56029 Defining the transcriptional signature of healing and non-healing lesions during murine cutaneous leishmaniasis

Data table header descriptions
ID_REF
VALUE quantile normalized signal
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 6798.6 0
ILMN_2721178 1403.4 0
ILMN_3033922 2684.4 0
ILMN_3092673 17906.6 0
ILMN_2816356 262.4 0.36216
ILMN_2808939 2196.9 0
ILMN_2634564 1423.4 0
ILMN_2737647 256.1 0.45865
ILMN_2734484 446.7 0
ILMN_2952292 789.5 0
ILMN_2699078 462.9 0
ILMN_1213681 1217.1 0
ILMN_2735413 223.3 0.901
ILMN_2735415 254.2 0.48371
ILMN_2891688 1395.4 0
ILMN_2637698 2759.2 0
ILMN_2674228 1246.8 0
ILMN_2601546 249.9 0.5589
ILMN_1230831 282.3 0.19173
ILMN_2848071 272.7 0.24687

Total number of rows: 25697

Table truncated, full table size 615 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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