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Sample GSM1350823 Query DataSets for GSM1350823
Status Public on Feb 19, 2019
Title B6.Lb_2
Sample type RNA
Source name whole mouse ear with Leishmania lesion
Organism Mus musculus
Characteristics strain of mouse: C57BL/6
species of leishmania: braziliensis
susceptibility phenotype: resistant
tissue: whole mouse ear
Treatment protocol Ear tissue was preserved in RNAlater reagent (Qiagen) and stored at -80c.
Growth protocol 6-8wk old mice were infected with 105 metacyclic-enriched parasites. Mice were killed at 4 weeks post-infection and the infected ear was recovered.
Extracted molecule total RNA
Extraction protocol RNAlater-preserved ear tissue was weighed, minced with a razor blade, and the entire ear was homogenized in 1ml of Qiazol (Qiagen) using 3 x 20 second pulses on a FastPrep-24 MP homogenizer (MP Biomedicals), with 5 min on ice between each pulse. Total RNA was isolated using the Qiagen miRNeasy kit.
Label biotin
Label protocol Biotin labeled complementary RNA (cRNA) was made using the EpiCentre Nano Total-Amp kit
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description 8942406069_E
Data processing The data were quantile normalized without background subtraction using Illumina GenomeStudio V1.8
Submission date Mar 19, 2014
Last update date Feb 19, 2019
Contact name daniel beiting
Organization name University of Pennsylvania
Department Pathobiology
Lab Beiting
Street address 380 S. University Ave
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
Platform ID GPL6885
Series (1)
GSE56029 Defining the transcriptional signature of healing and non-healing lesions during murine cutaneous leishmaniasis

Data table header descriptions
VALUE quantile normalized signal
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 7490.5 0
ILMN_2721178 1313.4 0
ILMN_3033922 2516 0
ILMN_3092673 21664.7 0
ILMN_2816356 323.4 0.02632
ILMN_2808939 1785.4 0
ILMN_2634564 1324.5 0
ILMN_2737647 282.5 0.13659
ILMN_2734484 619.2 0
ILMN_2952292 696.9 0
ILMN_2699078 426.5 0
ILMN_1213681 1314.2 0
ILMN_2735413 287 0.11028
ILMN_2735415 319.6 0.02882
ILMN_2891688 1735.2 0
ILMN_2637698 4137.9 0
ILMN_2674228 992.4 0
ILMN_2601546 288.3 0.10401
ILMN_1230831 266.3 0.31454
ILMN_2848071 309.3 0.04887

Total number of rows: 25697

Table truncated, full table size 613 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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