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Sample GSM1350825 Query DataSets for GSM1350825
Status Public on Feb 19, 2019
Title B6.Lb_4
Sample type RNA
 
Source name whole mouse ear with Leishmania lesion
Organism Mus musculus
Characteristics strain of mouse: C57BL/6
species of leishmania: braziliensis
susceptibility phenotype: resistant
tissue: whole mouse ear
Treatment protocol Ear tissue was preserved in RNAlater reagent (Qiagen) and stored at -80c.
Growth protocol 6-8wk old mice were infected with 105 metacyclic-enriched parasites. Mice were killed at 4 weeks post-infection and the infected ear was recovered.
Extracted molecule total RNA
Extraction protocol RNAlater-preserved ear tissue was weighed, minced with a razor blade, and the entire ear was homogenized in 1ml of Qiazol (Qiagen) using 3 x 20 second pulses on a FastPrep-24 MP homogenizer (MP Biomedicals), with 5 min on ice between each pulse. Total RNA was isolated using the Qiagen miRNeasy kit.
Label biotin
Label protocol Biotin labeled complementary RNA (cRNA) was made using the EpiCentre Nano Total-Amp kit
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description 8942406069_G
Data processing The data were quantile normalized without background subtraction using Illumina GenomeStudio V1.8
 
Submission date Mar 19, 2014
Last update date Feb 19, 2019
Contact name daniel beiting
E-mail(s) beiting@vet.upenn.edu
Organization name University of Pennsylvania
Department Pathobiology
Lab Beiting
Street address 380 S. University Ave
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
 
Platform ID GPL6885
Series (1)
GSE56029 Defining the transcriptional signature of healing and non-healing lesions during murine cutaneous leishmaniasis

Data table header descriptions
ID_REF
VALUE quantile normalized signal
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 7260.1 0
ILMN_2721178 1620 0
ILMN_3033922 2576.3 0
ILMN_3092673 17209.7 0
ILMN_2816356 334.7 0.01629
ILMN_2808939 1558.1 0
ILMN_2634564 1708 0
ILMN_2737647 301.2 0.07393
ILMN_2734484 573.3 0
ILMN_2952292 680.2 0
ILMN_2699078 353 0.00627
ILMN_1213681 1231.8 0
ILMN_2735413 271.5 0.26566
ILMN_2735415 314.1 0.04386
ILMN_2891688 1349.7 0
ILMN_2637698 3844.4 0
ILMN_2674228 1031.6 0
ILMN_2601546 307.3 0.0589
ILMN_1230831 252.8 0.51003
ILMN_2848071 291.3 0.11153

Total number of rows: 25697

Table truncated, full table size 611 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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