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Sample GSM1350833 Query DataSets for GSM1350833
Status Public on Feb 19, 2019
Title B6.Lm_2
Sample type RNA
 
Source name whole mouse ear with Leishmania lesion
Organism Mus musculus
Characteristics strain of mouse: C57BL/6
species of leishmania: major
susceptibility phenotype: resistant
tissue: whole mouse ear
Treatment protocol Ear tissue was preserved in RNAlater reagent (Qiagen) and stored at -80c.
Growth protocol 6-8wk old mice were infected with 105 metacyclic-enriched parasites. Mice were killed at 4 weeks post-infection and the infected ear was recovered.
Extracted molecule total RNA
Extraction protocol RNAlater-preserved ear tissue was weighed, minced with a razor blade, and the entire ear was homogenized in 1ml of Qiazol (Qiagen) using 3 x 20 second pulses on a FastPrep-24 MP homogenizer (MP Biomedicals), with 5 min on ice between each pulse. Total RNA was isolated using the Qiagen miRNeasy kit.
Label biotin
Label protocol Biotin labeled complementary RNA (cRNA) was made using the EpiCentre Nano Total-Amp kit
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description 8942406069_C
Data processing The data were quantile normalized without background subtraction using Illumina GenomeStudio V1.8
 
Submission date Mar 19, 2014
Last update date Feb 19, 2019
Contact name daniel beiting
E-mail(s) beiting@vet.upenn.edu
Organization name University of Pennsylvania
Department Pathobiology
Lab Beiting
Street address 380 S. University Ave
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
 
Platform ID GPL6885
Series (1)
GSE56029 Defining the transcriptional signature of healing and non-healing lesions during murine cutaneous leishmaniasis

Data table header descriptions
ID_REF
VALUE quantile normalized signal
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 6805.6 0
ILMN_2721178 1270.9 0
ILMN_3033922 2502.7 0
ILMN_3092673 17521.7 0
ILMN_2816356 328.3 0.01378
ILMN_2808939 1633.3 0
ILMN_2634564 1307.9 0
ILMN_2737647 276.5 0.17794
ILMN_2734484 527.7 0
ILMN_2952292 565.1 0
ILMN_2699078 411.4 0
ILMN_1213681 1180.7 0
ILMN_2735413 264.3 0.2995
ILMN_2735415 285.1 0.12782
ILMN_2891688 2088.5 0
ILMN_2637698 3713.5 0
ILMN_2674228 914.4 0
ILMN_2601546 280.6 0.15163
ILMN_1230831 271.5 0.22055
ILMN_2848071 302.1 0.05514

Total number of rows: 25697

Table truncated, full table size 608 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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