|
| Status |
Public on Feb 19, 2019 |
| Title |
B6.Lm_4 |
| Sample type |
RNA |
| |
|
| Source name |
whole mouse ear with Leishmania lesion
|
| Organism |
Mus musculus |
| Characteristics |
strain of mouse: C57BL/6
species of leishmania: major
susceptibility phenotype: resistant
tissue: whole mouse ear
|
| Treatment protocol |
Ear tissue was preserved in RNAlater reagent (Qiagen) and stored at -80c.
|
| Growth protocol |
6-8wk old mice were infected with 105 metacyclic-enriched parasites. Mice were killed at 4 weeks post-infection and the infected ear was recovered.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAlater-preserved ear tissue was weighed, minced with a razor blade, and the entire ear was homogenized in 1ml of Qiazol (Qiagen) using 3 x 20 second pulses on a FastPrep-24 MP homogenizer (MP Biomedicals), with 5 min on ice between each pulse. Total RNA was isolated using the Qiagen miRNeasy kit.
|
| Label |
biotin
|
| Label protocol |
Biotin labeled complementary RNA (cRNA) was made using the EpiCentre Nano Total-Amp kit
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
8942406069_B
|
| Data processing |
The data were quantile normalized without background subtraction using Illumina GenomeStudio V1.8
|
| |
|
| Submission date |
Mar 19, 2014 |
| Last update date |
Feb 19, 2019 |
| Contact name |
daniel beiting |
| E-mail(s) |
beiting@vet.upenn.edu
|
| Organization name |
University of Pennsylvania
|
| Department |
Pathobiology
|
| Lab |
Beiting
|
| Street address |
380 S. University Ave
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE56029 |
Defining the transcriptional signature of healing and non-healing lesions during murine cutaneous leishmaniasis |
|
