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Sample GSM135328 Query DataSets for GSM135328
Status Public on Feb 08, 2007
Title Celegans_gacA_4hours_RepA
Sample type RNA
 
Source name Celegans_gacA_4hours
Organism Caenorhabditis elegans
Characteristics Strain: fer-15(b26);fem-1(hc17)
Age: young adult
Tissue: whole animal
Treatment protocol Animals were transferred to P. aeruginosa PA14 mutant strain gacA for 4 hours prior to harvest.
Growth protocol fer-15;fem-1 animals were synchronized by hypochlorite treatment and first larval stage (L1) arrest. Arrested L1s were placed onto NGM plates seeded with OP50 and grown at the restrictive temperature (25°C) in order to obtain sterile adults. After ~52 hours of growth at 25°C (young adulthood), animals were transferred to SK plates seeded either with OP50, wild-type PA14 or gacA mutant PA14. Animals were harvested at 4 hours and 8 hours after transfer.
Extracted molecule total RNA
Extraction protocol TRI Reagent (Molecular Research Center)
Label biotin
Label protocol Affymetrix
 
Hybridization protocol Affymetrix
Scan protocol Affymetrix
Description fer-15;fem-1 animals were synchronized by hypochlorite treatment and first larval stage (L1) arrest. Arrested L1s were placed onto NGM plates seeded with OP50 and grown at the restrictive temperature (25°C) in order to obtain sterile adults. After ~52 hours of growth at 25°C (young adulthood), animals were transferred to SK plates seeded either with OP50, wild-type PA14 or gacA mutant PA14. Animals were harvested at 4 hours and 8 hours after transfer.
Data processing Resolver Gene Expression Data Analysis System, version 5.1 (Rosetta Inpharmatics)
 
Submission date Sep 07, 2006
Last update date Jun 23, 2008
Contact name Emily Troemel
E-mail(s) etroemel@ucsd.edu
Organization name University of California, San Diego
Department Cell and Developmental Biology
Lab Troemel
Street address 9500 Gilman Dr, #0349
City La Jolla
State/province CA
ZIP/Postal code 92093
Country USA
 
Platform ID GPL200
Series (1)
GSE5793 C. elegans gene expression in response to the pathogenic P. aeruginosa strain PA14.

Data table header descriptions
ID_REF
VALUE Intensity

Data table
ID_REF VALUE
171720_x_at 462.91925
171721_x_at 1026.55200
171722_x_at 1122.95935
171723_x_at 17436.73242
171724_x_at 1930.79834
171725_x_at 13979.09473
171726_x_at 100.23450
171727_x_at 23.95782
171728_x_at 85.44828
171729_x_at 600.93616
171730_x_at -7.41234
171731_x_at 1513.51147
171732_x_at 686.20990
171733_x_at 165.64116
171734_x_at 6549.64990
171735_x_at 4817.92969
171736_x_at 314.22101
171737_x_at 619.12402
171738_x_at 807.31042
171739_x_at 139.28433

Total number of rows: 22625

Table truncated, full table size 445 Kbytes.




Supplementary file Size Download File type/resource
GSM135328.CEL.gz 3.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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