|
| Status |
Public on Feb 08, 2007 |
| Title |
daf2daf16_RepB |
| Sample type |
RNA |
| |
|
| Source name |
daf2daf16
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
Strain: daf-2(e1368);daf-16(mgDf47)
Age: late L4/young adult
Tissue: whole animal
|
| Growth protocol |
Animals were synchronized by hypochlorite treatment and first larval stage (L1) arrest. Arrested L1s were placed onto NGM plates seeded with OP50 and grown at the permissive temperature (15°C) until L4, shifted to the restrictive temperature (25°C) for 6 hours and then harvested.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRI Reagent (Molecular Research Center)
|
| Label |
biotin
|
| Label protocol |
Affymetrix
|
| |
|
| Hybridization protocol |
Affymetrix
|
| Scan protocol |
Affymetrix
|
| Description |
Animals were synchronized by hypochlorite treatment and first larval stage (L1) arrest. Arrested L1s were placed onto NGM plates seeded with OP50 and grown at the permissive temperature (15°C) until L4, shifted to the restrictive temperature (25°C) for 6 hours and then harvested. daf-2;daf-16 animals are very slightly younger than daf-2 animals in RepB.
|
| Data processing |
Resolver Gene Expression Data Analysis System, version 5.1 (Rosetta Inpharmatics)
|
| |
|
| Submission date |
Sep 08, 2006 |
| Last update date |
Jun 23, 2008 |
| Contact name |
Emily Troemel |
| E-mail(s) |
etroemel@ucsd.edu
|
| Organization name |
University of California, San Diego
|
| Department |
Cell and Developmental Biology
|
| Lab |
Troemel
|
| Street address |
9500 Gilman Dr, #0349
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
|
| Platform ID |
GPL200 |
| Series (1) |
| GSE5801 |
Genes regulated by PMK-1 and DAF-16 in a daf-2(e1368) background. |
|
