|
Status |
Public on Apr 27, 2016 |
Title |
ERRγfl/fl mouse pancreatic islets infected with adenovirus Cre, technical replicate 1 |
Sample type |
RNA |
|
|
Source name |
pancreatic islets
|
Organism |
Mus musculus |
Characteristics |
genotype: ERRγ KO tissue: pancreatic islets genetic background: C57BL/6
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
technical replicate 1
|
Data processing |
The data were normalized using cubic spline in Illumina GenomeStudio and also using GeneSpring GX with normalizations set to the 75th percentile, and per-gene normalizations to the median and specific samples.
|
|
|
Submission date |
Mar 20, 2014 |
Last update date |
Apr 27, 2016 |
Contact name |
Ruth T Yu |
Organization name |
Salk Institute
|
Department |
Gene Expression Lab
|
Lab |
Ronald Evans
|
Street address |
10010 N Torrey Pines Rd
|
City |
La Jolla |
State/province |
CA |
ZIP/Postal code |
92037 |
Country |
USA |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE56080 |
Orphan Nuclear Receptor ERRγ is required for pancreatic islet beta-cell maturation |
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