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Sample GSM135478 Query DataSets for GSM135478
Status Public on Feb 08, 2007
Title daf2pmk1_RepA
Sample type RNA
 
Source name daf2pmk1
Organism Caenorhabditis elegans
Characteristics Strain: daf-2(e1368);pmk-1(km25)
Age: late L4/young adult
Tissue: whole animal
Growth protocol Animals were synchronized by hypochlorite treatment and first larval stage (L1) arrest. Arrested L1s were placed onto NGM plates seeded with OP50 and grown at the permissive temperature (15°C) until L4, shifted to the restrictive temperature (25°C) for 6 hours and then harvested.
Extracted molecule total RNA
Extraction protocol TRI Reagent (Molecular Research Center)
Label biotin
Label protocol Affymetrix
 
Hybridization protocol Affymetrix
Scan protocol Affymetrix
Description Animals were synchronized by hypochlorite treatment and first larval stage (L1) arrest. Arrested L1s were placed onto NGM plates seeded with OP50 and grown at the permissive temperature (15°C) until L4, shifted to the restrictive temperature (25°C) for 6 hours and then harvested.
Data processing Resolver Gene Expression Data Analysis System, version 5.1 (Rosetta Inpharmatics)
 
Submission date Sep 08, 2006
Last update date Jun 23, 2008
Contact name Emily Troemel
E-mail(s) etroemel@ucsd.edu
Organization name University of California, San Diego
Department Cell and Developmental Biology
Lab Troemel
Street address 9500 Gilman Dr, #0349
City La Jolla
State/province CA
ZIP/Postal code 92093
Country USA
 
Platform ID GPL200
Series (1)
GSE5801 Genes regulated by PMK-1 and DAF-16 in a daf-2(e1368) background.

Data table header descriptions
ID_REF
VALUE Intensity

Data table
ID_REF VALUE
171720_x_at 169.27124
171721_x_at 472.94446
171722_x_at 867.70032
171723_x_at 12950.93555
171724_x_at 1010.65308
171725_x_at 13385.92969
171726_x_at 45.88760
171727_x_at -0.26594
171728_x_at 88.17972
171729_x_at 431.82697
171730_x_at 44.93790
171731_x_at 678.90527
171732_x_at 435.66922
171733_x_at 8.58387
171734_x_at 4113.79346
171735_x_at 10180.84375
171736_x_at 59.31637
171737_x_at 187.09764
171738_x_at 696.01813
171739_x_at 181.85522

Total number of rows: 22625

Table truncated, full table size 441 Kbytes.




Supplementary file Size Download File type/resource
GSM135478.CEL.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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