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Status |
Public on Sep 12, 2006 |
Title |
Female BALB/c Lacrimal Placebo, biological rep 1 (Affymetrix) |
Sample type |
RNA |
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|
Source name |
Lacrimal glands from placebo treated female BALB/c mice ( glands pooled from 5-6 mice).
|
Organism |
Mus musculus |
Characteristics |
Female BALB/c Lacrimal Placebo, biological rep 1
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was purified with Trizol and RNAqueous spin columns following the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Codelink protocol from 2 microg total RNA using a CodeLink Expression Assay Reagent Kit.
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|
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Hybridization protocol |
Following fragmentation, 20 microg of cRNA were hybridized for 18 hr at 45C on a Affymetrix GeneChip Mouse Expression Array 430A using a Affymetrix hybridization oven. GeneChips were washed and stained with streptavidin-Alexa 647 and washed again using a Model 450 Fluidics station and Affymetrix GeneChip Operating Software (GCOS) .
|
Scan protocol |
GeneChips were scanned with a Affymetrix Model 7000 scanner running GCOS v1.3 software.
|
Description |
Lacrimal gland gene expression from ovarectomized mice treated with placebo for 14 days.
|
Data processing |
Data were analyzed with GCOS software using the default settings. No normalization was performed.
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Submission date |
Sep 11, 2006 |
Last update date |
Mar 18, 2009 |
Contact name |
Nathaniel Treister |
E-mail(s) |
ntreister@partners.org
|
Phone |
617-912-0298
|
Organization name |
Schepens Eye Research Institute
|
Department |
|
Lab |
Sullivan Lab
|
Street address |
20 Staniford Street
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02114 |
Country |
USA |
|
|
Platform ID |
GPL8321 |
Series (1) |
GSE3995 |
Female BALB/c Mouse Testosterone-Treated Submandibular/Lacrimal/Meibomian Glands |
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