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Sample GSM135568 Query DataSets for GSM135568
Status Public on Sep 12, 2006
Title Female BALB/c Lacrimal Testosterone, biological rep 2 (Affymetrix)
Sample type RNA
 
Source name Lacrimal glands from testosterone treated female BALB/c mice ( glands pooled from 5-6 mice).
Organism Mus musculus
Characteristics Female BALB/c Lacrimal Testosterone, biological rep 2
Extracted molecule total RNA
Extraction protocol RNA was purified with Trizol and RNAqueous spin columns following the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Codelink protocol from 2 microg total RNA using a CodeLink Expression Assay Reagent Kit.
 
Hybridization protocol Following fragmentation, 20 microg of cRNA were hybridized for 18 hr at 45C on a Affymetrix GeneChip Mouse Expression Array 430A using a Affymetrix hybridization oven. GeneChips were washed and stained with streptavidin-Alexa 647 and washed again using a Model 450 Fluidics station and Affymetrix GeneChip Operating Software (GCOS) .
Scan protocol GeneChips were scanned with a Affymetrix Model 7000 scanner running GCOS v1.3 software.
Description Lacrimal gland gene expression from ovarectomized mice treated with testosterone for 14 days.
Data processing Data were analyzed with GCOS software using the default settings. No normalization was performed.
 
Submission date Sep 11, 2006
Last update date Mar 18, 2009
Contact name Nathaniel Treister
E-mail(s) ntreister@partners.org
Phone 617-912-0298
Organization name Schepens Eye Research Institute
Department
Lab Sullivan Lab
Street address 20 Staniford Street
City Boston
State/province MA
ZIP/Postal code 02114
Country USA
 
Platform ID GPL8321
Series (1)
GSE3995 Female BALB/c Mouse Testosterone-Treated Submandibular/Lacrimal/Meibomian Glands

Data table header descriptions
ID_REF
VALUE
ABS_CALL

Data table
ID_REF VALUE ABS_CALL
AFFX-BioB-5_at 110.4 P
AFFX-BioB-M_at 204.8 P
AFFX-BioB-3_at 167.5 P
AFFX-BioC-5_at 393.2 P
AFFX-BioC-3_at 481.1 P
AFFX-BioDn-5_at 891 P
AFFX-BioDn-3_at 1909.8 P
AFFX-CreX-5_at 5147.3 P
AFFX-CreX-3_at 6674.5 P
AFFX-DapX-5_at 2.5 A
AFFX-DapX-M_at 3.2 A
AFFX-DapX-3_at 1.7 A
AFFX-LysX-5_at 4.2 A
AFFX-LysX-M_at 1.2 A
AFFX-LysX-3_at 2.5 A
AFFX-PheX-5_at 0.5 A
AFFX-PheX-M_at 1.3 A
AFFX-PheX-3_at 1.2 A
AFFX-ThrX-5_at 0.9 A
AFFX-ThrX-M_at 3.4 A

Total number of rows: 22690

Table truncated, full table size 404 Kbytes.




Supplementary file Size Download File type/resource
GSM135568.CEL.gz 1.7 Mb (ftp)(http) CEL

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