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Sample GSM135702 Query DataSets for GSM135702
Status Public on Jul 01, 2007
Title Sepsis patient (sample V7)
Sample type RNA
 
Channel 1
Source name Neutrophil
Organism Homo sapiens
Characteristics Sepsis Patient
Biomaterial provider Nepean Hospital Intensive Care Unit
Extracted molecule total RNA
Extraction protocol RiboPure-Blood kit (Ambion)
Label Cy5
Label protocol SuperScriptâ„¢ Plus Indirect cDNA Labeling System for DNA Microarrays (Invitrogen)
 
Channel 2
Source name Cell line
Organism Homo sapiens
Characteristics Universal Reference RNA
Biomaterial provider Stratagene
Extracted molecule total RNA
Label Cy3
Label protocol SuperScriptâ„¢ Plus Indirect cDNA Labeling System for DNA Microarrays (Invitrogen)
 
 
Hybridization protocol SuperScriptâ„¢ Plus Indirect cDNA Labeling System for DNA Microarrays (Invitrogen)
Scan protocol GenePix 4000B laser scanner and GenePix software(Axon Instruments)
Description This sample is included in the validation set
Data processing Background subtraction followed by Lowess print-tip group Normalization, include genes which have (1). at least 80% of expression data with 2 fold change in either direction from the gene's median value, (2) at least 50% of expression data non-missing
 
Submission date Sep 11, 2006
Last update date Jun 15, 2007
Contact name Benjamin Man Piu Tang
E-mail(s) benjamin@clubsalsa.com.au
Phone 61415614883
Organization name Nepean Hospital
Department Intensive Care Medicine
Street address 12/29 Cook Street
City Glebe
State/province NSW
ZIP/Postal code 2037
Country Australia
 
Platform ID GPL4274
Series (1)
GSE5772 Leukocytes from patients with sepsis

Data table header descriptions
ID_REF
VALUE Log (Cy5/Cy3)

Data table
ID_REF VALUE
AB000464 0.493101656
AB000509 -0.100255892
AB000520 NULL
AB000895 NULL
AB001328 0.271592766
AB001451 0.137379721
AB002282 -2.871333122
AB002293 -0.125069708
AB002296 -0.106105842
AB002297 0.320503831
AB002298 -0.336241513
AB002299 -0.22693868
AB002301 -0.078558035
AB002304 -0.006918833
AB002306 -0.164899901
AB002308 0.35686934
AB002313 -0.295414418
AB002314 -0.241060868
AB002316 -0.547844291
AB002318 -0.375305802

Total number of rows: 18664

Table truncated, full table size 385 Kbytes.




Supplementary file Size Download File type/resource
GSM135702.GPR.gz 2.0 Mb (ftp)(http) GPR
Processed data included within Sample table

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