|
| Status |
Public on Nov 19, 2010 |
| Title |
1 mt E11.5 heart |
| Sample type |
RNA |
| |
|
| Source name |
skNAC knockout heart
|
| Organism |
Mus musculus |
| Characteristics |
strain: 129SvEv
developmental stage: E11.5
tissue: ventricle
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
Biotin
|
| Label protocol |
500ng of total RNA was amplified by using MessageAmpTM-Biotin Enhanced Kit from Ambion
|
| |
|
| Hybridization protocol |
Generic Affymetrix Eukaryotic Array
|
| Scan protocol |
Generic Affymetrix Eukaryotic Array
|
| Description |
Targeted deletion of skNAC in mice rsulted in embryonic lethality with cardiac defects by E12.5. In order to investigate the molecular mechanism of the cardiac defect, we compared gene expression profiles of the knockout heart and wild type heart.
|
| Data processing |
GCOS (MAS5)
|
| |
|
| Submission date |
Sep 11, 2006 |
| Last update date |
Nov 19, 2010 |
| Contact name |
Chong Yon Park |
| E-mail(s) |
cpark@gladstone.ucsf.edu
|
| Phone |
415-734-2806
|
| Organization name |
Gladstone Institute
|
| Department |
Cardiovascular Diseases
|
| Lab |
Deepak Srivastava
|
| Street address |
1650 Owens St.
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE5841 |
Differential gene expression in mouse skNAC knockout heart at E11.5 |
|
