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Sample GSM135757 Query DataSets for GSM135757
Status Public on Nov 19, 2010
Title 26 mt E11.5 heart
Sample type RNA
 
Source name skNAC knockout heart
Organism Mus musculus
Characteristics strain: 129SvEv
developmental stage: E11.5
tissue: ventricle
Extracted molecule total RNA
Extraction protocol Trizol
Label Biotin
Label protocol 500ng of total RNA was amplified by using MessageAmpTM-Biotin Enhanced Kit from Ambion
 
Hybridization protocol Generic Affymetrix Eukaryotic Array
Scan protocol Generic Affymetrix Eukaryotic Array
Description Targeted deletion of skNAC in mice rsulted in embryonic lethality with cardiac defects by E12.5. In order to investigate the molecular mechanism of the cardiac defect, we compared gene expression profiles of the knockout heart and wild type heart.
Data processing GCOS (MAS5)
 
Submission date Sep 11, 2006
Last update date Nov 19, 2010
Contact name Chong Yon Park
E-mail(s) cpark@gladstone.ucsf.edu
Phone 415-734-2806
Organization name Gladstone Institute
Department Cardiovascular Diseases
Lab Deepak Srivastava
Street address 1650 Owens St.
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
 
Platform ID GPL1261
Series (1)
GSE5841 Differential gene expression in mouse skNAC knockout heart at E11.5

Data table header descriptions
ID_REF
VALUE skNAC knockout mouse ventricle - sample 26
ABS_CALL skNAC knockout mouse ventricle - sample 26

Data table
ID_REF VALUE ABS_CALL
AFFX-BioB-5_at 233.3 P
AFFX-BioB-M_at 296.9 P
AFFX-BioB-3_at 170.1 P
AFFX-BioC-5_at 472.2 P
AFFX-BioC-3_at 368.2 P
AFFX-BioDn-5_at 542.1 P
AFFX-BioDn-3_at 2659.5 P
AFFX-CreX-5_at 4520.4 P
AFFX-CreX-3_at 7224.7 P
AFFX-DapX-5_at 45.6 A
AFFX-DapX-M_at 21.8 A
AFFX-DapX-3_at 43 A
AFFX-LysX-5_at 2.8 A
AFFX-LysX-M_at 29 A
AFFX-LysX-3_at 5.9 A
AFFX-PheX-5_at 7.4 A
AFFX-PheX-M_at 10.1 A
AFFX-PheX-3_at 15 A
AFFX-ThrX-5_at 30.2 A
AFFX-ThrX-M_at 18.9 A

Total number of rows: 45101

Table truncated, full table size 822 Kbytes.




Supplementary file Size Download File type/resource
GSM135757.CEL.gz 6.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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