|
| Status |
Public on Mar 28, 2014 |
| Title |
SIRT4 KO liver_7245 |
| Sample type |
RNA |
| |
|
| Source name |
SIRT4 KO mouse liver after 16 hrs fast
|
| Organism |
Mus musculus |
| Characteristics |
strain: 129SV
gender: male
genotype/variation: SIRT4 KO
tissue: liver
|
| Treatment protocol |
No treatment. Samples are from SIRT4 WT or KO littermate mice on a 129 background.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to manufacturer instructions (Invitrogen). RNA was cleaned using RNeasy MiniKit from Qiagen.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip mouse Genome Array Mouse430_2. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression from Ko liver
|
| Data processing |
The data were analyzed with Robust Multi-array Average (RMA) with PM only models as normalization method.
|
| |
|
| Submission date |
Mar 28, 2014 |
| Last update date |
Mar 28, 2014 |
| Contact name |
Gaelle Laurent |
| E-mail(s) |
gaelle_laurent@hms.harvard.edu
|
| Organization name |
Harvard Medical School
|
| Department |
Cell Biology
|
| Lab |
Marcia Haigis
|
| Street address |
250 Longwood Avenue
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
|
