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Sample GSM1359243 Query DataSets for GSM1359243
Status Public on Mar 28, 2014
Title G1S_2
Sample type RNA
Source name FL-HSPCs, transduced with LeGO-iG-Gata1s/shG1, grown 4 days in myeloid differentiation medium, duplicate 2
Organism Homo sapiens
Characteristics cell line: wild-type CD34+-hematopoietic stem and progenitor cells
genotype/variation (vector): LeGO-iG-Gata1s/shG1
days: 4
Treatment protocol FL-HSPCs were transduced with the indicated virus.
Growth protocol FL-HSPCs cells were grown in RPMI, 20% FCS, 1% P/S, SCF (5ng/ml), GM-CSF (10ng/ml), G-SCF (10ng/ml), IL-3 (5ng/ml)
Extracted molecule total RNA
Extraction protocol RNeasy Mini Kit (Qiagen)
Label Cy3
Label protocol Cyanine-3 (Cy3) labeled cRNA was prepared from 0.5 ug RNA using the One-Color Low RNA Input Linear Amplification PLUS kit (Agilent) according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN, Valencia, CA). Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.
Hybridization protocol 1.5 ug of Cy3-labelled cRNA (specific activity >10.0 pmol Cy3/ug cRNA) was fragmented at 60°C for 30 minutes in a reaction volume of 250 ml containing 1x Agilent fragmentation buffer and 2x Agilent blocking agent following the manufacturers instructions. On completion of the fragmentation reaction, 250 ml of 2x Agilent hybridization buffer was added to the fragmentation mixture and hybridized to Agilent Whole Human Genome Oligo Microarrays (G4112A) for 17 hours at 65°C in a rotating Agilent hybridization oven. After hybridization, microarrays were washed 1 minute at room temperature with GE Wash Buffer 1 (Agilent) and 1 minute with 37°C GE Wash buffer 2 (Agilent), then dried immediately by brief centrifugation.
Scan protocol Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505B) using one color scan setting for 1x44k array slides (Scan Area 61x21.6 mm, Scan resolution 10um, Dye channel is set to Green and Green PMT is set to 100%).
Data processing The scanned images were analyzed with Feature Extraction Software 10.5 (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
Submission date Mar 28, 2014
Last update date Mar 28, 2014
Contact name Jan-Henning Klusmann
Organization name Goehte University Frankfurt
Department Pediatric Hematology and Oncology
Street address Theodor-Stern-Kai 7
City Frankfurt
ZIP/Postal code 60590
Country Germany
Platform ID GPL6480
Series (1)
GSE56332 GATA1s induces hyperproliferation of eosinophil precursors in Down syndrome transient leukemia

Data table header descriptions
VALUE log2 transformed, scaled to mean of all samples

Data table
GE_BrightCorner 16.29464
DarkCorner 3.00145
A_24_P66027 8.584753
A_32_P77178 2.921588
A_23_P212522 8.308174
A_24_P934473 5.6621556
A_24_P9671 12.074968
A_32_P29551 2.9229932
A_24_P801451 6.8583827
A_32_P30710 16.180653
A_32_P89523 2.9241102
A_24_P704878 2.9246824
A_32_P86028 16.536934
A_24_P470079 6.07616
A_23_P65830 11.072788
A_23_P109143 12.922116
A_24_P595567 5.289281
A_24_P391591 9.904998
A_24_P799245 2.9317935
A_24_P932757 3.19614

Total number of rows: 41093

Table truncated, full table size 891 Kbytes.

Supplementary file Size Download File type/resource
GSM1359243_251485076630_Emmrich7012_S01_GE1_107_Sep09_1_4.txt.gz 2.2 Mb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table

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