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Sample GSM1359767 Query DataSets for GSM1359767
Status Public on Mar 31, 2014
Title VCAP DMSO vehicle at 24 hrs, Biological rep2
Sample type RNA
 
Source name VCAP DMSO vehicle at 24 hrs
Organism Homo sapiens
Characteristics cell line: Human cancer cell line, VCAP
dose: vehicle
time: 24 hrs
Treatment protocol Prostate cell lines were treated with DMSO or BET inhibitor GSK525762A (0.5 or 10 uM) at 24 hours
Extracted molecule total RNA
Extraction protocol PolyA mRNA was extracted according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug PolyA mRNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from VCAP DMSO vehicle at 24 hrs
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Mar 31, 2014
Last update date Mar 31, 2014
Contact name Yuchen Bai
E-mail(s) yuchen.v.bai@gsk.com
Phone 610-917-4981
Organization name GlaxoSmithKline
Street address 1250 S Collegeville Road
City Collegeville
State/province PA
ZIP/Postal code 19426
Country USA
 
Platform ID GPL570
Series (1)
GSE56352 Inhibition of BET bromodomain proteins as a therapeutic approach in prostate cancer

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
1007_s_at 2509.508734
1053_at 1421.459769
117_at 49.07311799
121_at 818.8071754
1255_g_at 14.79403266
1294_at 85.97545691
1316_at 66.35043482
1320_at 55.09611748
1405_i_at 6.520189814
1431_at 51.13718174
1438_at 265.7647238
1487_at 1356.173365
1494_f_at 101.5238382
1552256_a_at 3055.081143
1552257_a_at 3905.260163
1552258_at 18.10942268
1552261_at 30.83782569
1552263_at 365.466133
1552264_a_at 1105.780776
1552266_at 7.213404639

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM1359767_EA10008_246211_HG-U133_PLUS_2_8.CEL.gz 5.0 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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