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Sample GSM1359768 Query DataSets for GSM1359768
Status Public on Mar 31, 2014
Title VCAP GSK525762A .5uM at 24 hrs, Biological rep1
Sample type RNA
 
Source name VCAP GSK525762A .5uM at 24 hrs
Organism Homo sapiens
Characteristics cell line: Human cancer cell line, VCAP
dose: .5uM
time: 24 hrs
Treatment protocol Prostate cell lines were treated with DMSO or BET inhibitor GSK525762A (0.5 or 10 uM) at 24 hours
Extracted molecule total RNA
Extraction protocol PolyA mRNA was extracted according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug PolyA mRNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from VCAP GSK525762A .5uM at 24 hrs
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Mar 31, 2014
Last update date Mar 31, 2014
Contact name Matteo Righi
E-mail(s) m.righi@autolus.com
Organization name Autolus Therapeutics
Street address The MediaWorks, 191 Wood Ln
City London
State/province London
ZIP/Postal code W12 7FP
Country United Kingdom
 
Platform ID GPL570
Series (1)
GSE56352 Inhibition of BET bromodomain proteins as a therapeutic approach in prostate cancer

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
1007_s_at 2395.462782
1053_at 732.4989493
117_at 71.61581046
121_at 855.2961909
1255_g_at 20.4090551
1294_at 75.35326231
1316_at 62.54669341
1320_at 34.0731819
1405_i_at 12.09249269
1431_at 74.47476517
1438_at 256.5667276
1487_at 1064.689243
1494_f_at 142.285243
1552256_a_at 1354.345819
1552257_a_at 3270.823375
1552258_at 79.3344882
1552261_at 6.409541468
1552263_at 389.9056679
1552264_a_at 1272.010677
1552266_at 13.02444512

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM1359768_EA10008_246212_HG-U133_PLUS_2_9.CEL.gz 5.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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