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Sample GSM1359771 Query DataSets for GSM1359771
Status Public on Mar 31, 2014
Title VCAP GSK525762A 10uM at 24 hrs, Biological rep2
Sample type RNA
 
Source name VCAP GSK525762A 10uM at 24 hrs
Organism Homo sapiens
Characteristics cell line: Human cancer cell line, VCAP
dose: 10uM
time: 24 hrs
Treatment protocol Prostate cell lines were treated with DMSO or BET inhibitor GSK525762A (0.5 or 10 uM) at 24 hours
Extracted molecule total RNA
Extraction protocol PolyA mRNA was extracted according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug PolyA mRNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from VCAP GSK525762A 10uM at 24 hrs
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Mar 31, 2014
Last update date Mar 31, 2014
Contact name Yuchen Bai
E-mail(s) yuchen.v.bai@gsk.com
Phone 610-917-4981
Organization name GlaxoSmithKline
Street address 1250 S Collegeville Road
City Collegeville
State/province PA
ZIP/Postal code 19426
Country USA
 
Platform ID GPL570
Series (1)
GSE56352 Inhibition of BET bromodomain proteins as a therapeutic approach in prostate cancer

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
1007_s_at 1713.78439
1053_at 769.1488109
117_at 58.82876765
121_at 1025.840544
1255_g_at 44.44126276
1294_at 64.91799136
1316_at 44.23322362
1320_at 117.9216441
1405_i_at 3.558639516
1431_at 58.01314569
1438_at 270.4813622
1487_at 1042.102344
1494_f_at 133.8922763
1552256_a_at 984.4491383
1552257_a_at 3626.027942
1552258_at 70.8308711
1552261_at 42.27525202
1552263_at 504.1738459
1552264_a_at 1277.700921
1552266_at 10.00679241

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM1359771_EA10008_246215_HG-U133_PLUS_2_12.CEL.gz 5.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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