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Sample GSM1359777 Query DataSets for GSM1359777
Status Public on Mar 31, 2014
Title LNCAP GSK525762A 10uM at 24 hrs, Biological rep2
Sample type RNA
 
Source name LNCAP GSK525762A 10uM at 24 hrs
Organism Homo sapiens
Characteristics cell line: Human cancer cell line, LNCAP
dose: 10uM
time: 24 hrs
Treatment protocol Prostate cell lines were treated with DMSO or BET inhibitor GSK525762A (0.5 or 10 uM) at 24 hours
Extracted molecule total RNA
Extraction protocol PolyA mRNA was extracted according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug PolyA mRNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from LNCAP GSK525762A 10uM at 24 hrs
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Mar 31, 2014
Last update date Mar 31, 2014
Contact name Yuchen Bai
E-mail(s) yuchen.v.bai@gsk.com
Phone 610-917-4981
Organization name GlaxoSmithKline
Street address 1250 S Collegeville Road
City Collegeville
State/province PA
ZIP/Postal code 19426
Country USA
 
Platform ID GPL570
Series (1)
GSE56352 Inhibition of BET bromodomain proteins as a therapeutic approach in prostate cancer

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
1007_s_at 1168.137387
1053_at 864.5292587
117_at 80.02655278
121_at 939.3702723
1255_g_at 36.96511323
1294_at 71.81727923
1316_at 77.76721262
1320_at 193.9939123
1405_i_at 30.72026636
1431_at 16.76002997
1438_at 608.3274661
1487_at 2066.515392
1494_f_at 171.3198402
1552256_a_at 1453.413927
1552257_a_at 3733.538278
1552258_at 49.80284146
1552261_at 7.407969067
1552263_at 397.9362682
1552264_a_at 944.2702384
1552266_at 13.94041518

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM1359777_EA10008_246221_HG-U133_PLUS_2_18.CEL.gz 5.0 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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