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Sample GSM1359781 Query DataSets for GSM1359781
Status Public on Mar 31, 2014
Title PC3 GSK525762A .5uM at 24 hrs, Biological rep2
Sample type RNA
 
Source name PC3 GSK525762A .5uM at 24 hrs
Organism Homo sapiens
Characteristics cell line: Human cancer cell line, PC3
dose: .5uM
time: 24 hrs
Treatment protocol Prostate cell lines were treated with DMSO or BET inhibitor GSK525762A (0.5 or 10 uM) at 24 hours
Extracted molecule total RNA
Extraction protocol PolyA mRNA was extracted according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug PolyA mRNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from PC3 GSK525762A .5uM at 24 hrs
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Mar 31, 2014
Last update date Mar 31, 2014
Contact name Yuchen Bai
E-mail(s) yuchen.v.bai@gsk.com
Phone 610-917-4981
Organization name GlaxoSmithKline
Street address 1250 S Collegeville Road
City Collegeville
State/province PA
ZIP/Postal code 19426
Country USA
 
Platform ID GPL570
Series (1)
GSE56352 Inhibition of BET bromodomain proteins as a therapeutic approach in prostate cancer

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity

Data table
ID_REF VALUE
1007_s_at 2792.915401
1053_at 1420.24565
117_at 57.69288271
121_at 758.2188748
1255_g_at 28.42896877
1294_at 89.50488861
1316_at 83.1162634
1320_at 138.4322144
1405_i_at 50.04123264
1431_at 118.0855427
1438_at 286.8112267
1487_at 1143.099901
1494_f_at 118.0271401
1552256_a_at 1568.453531
1552257_a_at 1216.173464
1552258_at 110.0329654
1552261_at 21.83719441
1552263_at 279.3490733
1552264_a_at 1518.432863
1552266_at 7.965924179

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM1359781_EA10008_248027_HG-U133_PLUS_2_103.CEL.gz 5.0 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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