|
| Status |
Public on Mar 31, 2014 |
| Title |
PC3 GSK525762A 10uM at 24 hrs, Biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
PC3 GSK525762A 10uM at 24 hrs
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Human cancer cell line, PC3
dose: 10uM
time: 24 hrs
|
| Treatment protocol |
Prostate cell lines were treated with DMSO or BET inhibitor GSK525762A (0.5 or 10 uM) at 24 hours
|
| Extracted molecule |
total RNA |
| Extraction protocol |
PolyA mRNA was extracted according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug PolyA mRNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from PC3 GSK525762A 10uM at 24 hrs
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Mar 31, 2014 |
| Last update date |
Mar 31, 2014 |
| Contact name |
Matteo Righi |
| E-mail(s) |
m.righi@autolus.com
|
| Organization name |
Autolus Therapeutics
|
| Street address |
The MediaWorks, 191 Wood Ln
|
| City |
London |
| State/province |
London |
| ZIP/Postal code |
W12 7FP |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE56352 |
Inhibition of BET bromodomain proteins as a therapeutic approach in prostate cancer |
|
