|
Status |
Public on Apr 01, 2014 |
Title |
control embryo: biological replicate 3 |
Sample type |
RNA |
|
|
Source name |
4 dpf dissected larval zebrafish trunks injected at 2 dpf with PBS
|
Organism |
Danio rerio |
Characteristics |
tissue: dissected larval zebrafish trunks (approx 50) treatment: injected at 2 dpf with PBS
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
Description |
biotin
|
Data processing |
The data were normalised with the RMA algorithym using default settings which included background correction.
|
|
|
Submission date |
Mar 31, 2014 |
Last update date |
Apr 01, 2014 |
Contact name |
Cris Print |
E-mail(s) |
c.print@auckland.ac.nz
|
Organization name |
Auckland University
|
Street address |
Grafton Campus, Park Rd
|
City |
Auckland |
ZIP/Postal code |
1 |
Country |
New Zealand |
|
|
Platform ID |
GPL1319 |
Series (1) |
GSE56365 |
Epidermal cells help coordinate leukocyte migration during inflammation through fatty acid-fueled matrix metalloproteinase production |
|