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Sample GSM1359954 Query DataSets for GSM1359954
Status Public on Apr 01, 2014
Title control embryo: biological replicate 3
Sample type RNA
 
Source name 4 dpf dissected larval zebrafish trunks injected at 2 dpf with PBS
Organism Danio rerio
Characteristics tissue: dissected larval zebrafish trunks (approx 50)
treatment: injected at 2 dpf with PBS
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description biotin
Data processing The data were normalised with the RMA algorithym using default settings which included background correction.
 
Submission date Mar 31, 2014
Last update date Apr 01, 2014
Contact name Cris Print
E-mail(s) c.print@auckland.ac.nz
Organization name Auckland University
Street address Grafton Campus, Park Rd
City Auckland
ZIP/Postal code 1
Country New Zealand
 
Platform ID GPL1319
Series (1)
GSE56365 Epidermal cells help coordinate leukocyte migration during inflammation through fatty acid-fueled matrix metalloproteinase production

Data table header descriptions
ID_REF
VALUE log base 2 signal intensity

Data table
ID_REF VALUE
AFFX-BioB-3_at 7.91203923
AFFX-BioB-5_at 8.2269181
AFFX-BioB-M_at 8.569483935
AFFX-BioC-3_at 9.531443595
AFFX-BioC-5_at 9.358889215
AFFX-BioDn-3_at 11.6796808
AFFX-BioDn-5_at 10.05594308
AFFX-CreX-3_at 12.58875829
AFFX-CreX-5_at 12.18363365
AFFX-DapX-3_at 8.133299735
AFFX-DapX-5_at 5.226428143
AFFX-DapX-M_at 7.328296839
AFFX-Dr-AB076373-1_at 4.279361002
AFFX-Dr-acta1-3_at 14.59330458
AFFX-Dr-acta1-5_at 12.20435061
AFFX-Dr-acta1-5_x_at 13.25617291
AFFX-Dr-acta1-M_at 13.70396624
AFFX-Dr-AF292559-1_at 3.323399478
AFFX-Dr-AF292559-2_s_at 2.44552728
AFFX-Dr-AF292559-3_s_at 2.746882347

Total number of rows: 15617

Table truncated, full table size 436 Kbytes.




Supplementary file Size Download File type/resource
GSM1359954_CH0005_Zebrafish_.CEL.gz 1.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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