|
| Status |
Public on Apr 01, 2014 |
| Title |
infected embryo: biological replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
4 dpf dissected larval zebrafish trunks injected at 2 dpf with live Salmonella enterica serovar Typhimurium bacteria
|
| Organism |
Danio rerio |
| Characteristics |
tissue: dissected larval zebrafish trunks (approx 50)
treatment: injected at 2 dpf with live Salmonella enterica serovar Typhimurium bacteria
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
biotin
|
| Data processing |
The data were normalised with the RMA algorithym using default settings which included background correction.
|
| |
|
| Submission date |
Mar 31, 2014 |
| Last update date |
Apr 01, 2014 |
| Contact name |
Cris Print |
| E-mail(s) |
c.print@auckland.ac.nz
|
| Organization name |
Auckland University
|
| Street address |
Grafton Campus, Park Rd
|
| City |
Auckland |
| ZIP/Postal code |
1 |
| Country |
New Zealand |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE56365 |
Epidermal cells help coordinate leukocyte migration during inflammation through fatty acid-fueled matrix metalloproteinase production |
|
