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Sample GSM1359979 Query DataSets for GSM1359979
Status Public on Sep 05, 2014
Title non receptive endometrium at day 3 of estrous cycle after in vivo embryo produce transfer pool1
Sample type RNA
 
Source name endometrium
Organism Bos taurus
Characteristics breed: Simmental
gender: heifers
time: day 3
type of transfer: in vivo
receptive: no
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using TRI Reagent (Sigma, Taufkirchen, Germany) according to the manufacturer’s protocol. After DNaseI treatment the RNA was cleaned up using the RNeasy Kit (Qiagen, Hilden, Germany).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 500 ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 42C on Affymetrix GeneChip Porcine Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol Scanning using the GeneChip scanner 3000 were performed according to Affymetrix protocols
Description miRNA expression data from endometrium
Data processing Data were analysed with Affymetrix GCOS 1.1.1 software using global scaling to a target signal of 500.
 
Submission date Mar 31, 2014
Last update date Sep 06, 2014
Contact name Klaus Wimmers
E-mail(s) s.wimmers@fbn-dummerstorf.de
Phone 0049 3820868700
Organization name Leibniz Institute for Farm Animal Biology (FBN)
Street address Wilhelm-Stahl-Allee 2
City Dummerstorf
ZIP/Postal code 18196
Country Germany
 
Platform ID GPL16384
Series (1)
GSE56368 miRNA profile of the bovine pretransfer endometrium based on pregnancy success after in vivo and in vitro produced embryos transfer

Data table header descriptions
ID_REF
VALUE by PLIER algorithm

Data table
ID_REF VALUE
14q0_st 1.52
14qI-1_st 4.14
14qI-1_x_st 3.19
14qI-2_st 0.85
14qI-3_x_st 1.34
14qI-4_st 0.69
14qI-4_x_st 1.20
14qI-5_st 1.36
14qI-6_st 1.35
14qI-6_x_st 1.56
14qI-7_st 1.22
14qI-8_st 1.61
14qI-8_x_st 1.37
14qI-9_x_st 1.38
14qII-10_st 1.13
14qII-10_x_st 0.98
14qII-11_st 1.29
14qII-11_x_st 0.60
14qII-12_st 1.06
14qII-12_x_st 0.94

Total number of rows: 25015

Table truncated, full table size 539 Kbytes.




Supplementary file Size Download File type/resource
GSM1359979_Endo.mi_viv_np_d3_pool_1.CEL.gz 837.2 Kb (ftp)(http) CEL
Processed data included within Sample table

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