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Sample GSM1361216 Query DataSets for GSM1361216
Status Public on Mar 24, 2015
Title WT_Saline_2h_rep2
Sample type RNA
 
Source name WT uterus, saline control
Organism Mus musculus
Characteristics esr1 genotype: WT
age: adult (10+ weeks)
gender: female (ovariectomized)
strain: C57bl6/Jx129
agent: control
tissue: uterus
Treatment protocol mice were injected with 100 ul saline of 100 ul E2 and uteri were collected 2 hours later and snap frozen in liquid nitrogen
Growth protocol WT and EAAE mice were ovariectomized and rested for 10-14 days to clear endogenous ovarian hormones
Extracted molecule total RNA
Extraction protocol frozen uterine tissue was pulverized and each uterus was homogenized in 800 ul of Trizol (Invitrogen) with 250 ug/ml glycogen. Extraction proceeded as descibed in manufacturer's protocol. Following resuspension of RNA pellet, a second preciptation was done RNA was DNAse treated and cleaned up using Rneasy kits
Label Cy3
Label protocol Starting with 500ng of total RNA, Cy3 labeled cRNA was produced according to manufacturer’s protocol.
 
Hybridization protocol For each sample, 1.65ug of Cy3 labeled cRNAs were fragmented and hybridized for 17 hours in a rotating hybridization oven.
Scan protocol Slides were washed and then scanned with an Agilent Scanner.
Description gene expression in WT ovariectomized saline vehicle treated uterus
Data processing Data was obtained using the Agilent Feature Extraction software (v9.5), using the 1-color defaults for all parameters.
 
Submission date Apr 01, 2014
Last update date Mar 25, 2015
Contact name NIEHS Microarray Core
E-mail(s) microarray@niehs.nih.gov, liuliw@niehs.nih.gov
Organization name NIEHS
Department DIR
Lab Microarray Core
Street address 111 T.W. Alexander Drive
City RTP
State/province NC
ZIP/Postal code 27709
Country USA
 
Platform ID GPL4134
Series (1)
GSE56423 Estradiol Induced Transcriptional Profile of EAAE DNA Binding Deficient ERa Uterus

Data table header descriptions
ID_REF
VALUE processed Cy3 signal intensity (Agilent gProcessedSignal)

Data table
ID_REF VALUE
1 6.045160e+004
2 6.350935e+000
3 6.368741e+000
4 6.384365e+000
5 6.397796e+000
6 6.409270e+000
7 6.419061e+000
8 6.426917e+000
9 6.433219e+000
10 6.436491e+000
11 6.438721e+000
12 6.439190e+000
13 2.139492e+002
14 2.276528e+001
15 8.941570e+001
16 2.649639e+003
18 1.133003e+001
19 6.398423e+000
20 9.575270e+001
21 6.729247e+001

Total number of rows: 45018

Table truncated, full table size 868 Kbytes.




Supplementary file Size Download File type/resource
GSM1361216_US23502387_251486839034_S01_GE1-v5_95_Feb07_1_1.txt.gz 9.2 Mb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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