|
| Status |
Public on Mar 24, 2015 |
| Title |
WT_Saline_2h_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
WT uterus, saline control
|
| Organism |
Mus musculus |
| Characteristics |
esr1 genotype: WT
age: adult (10+ weeks)
gender: female (ovariectomized)
strain: C57bl6/Jx129
agent: control
tissue: uterus
|
| Treatment protocol |
mice were injected with 100 ul saline of 100 ul E2 and uteri were collected 2 hours later and snap frozen in liquid nitrogen
|
| Growth protocol |
WT and EAAE mice were ovariectomized and rested for 10-14 days to clear endogenous ovarian hormones
|
| Extracted molecule |
total RNA |
| Extraction protocol |
frozen uterine tissue was pulverized and each uterus was homogenized in 800 ul of Trizol (Invitrogen) with 250 ug/ml glycogen. Extraction proceeded as descibed in manufacturer's protocol. Following resuspension of RNA pellet, a second preciptation was done RNA was DNAse treated and cleaned up using Rneasy kits
|
| Label |
Cy3
|
| Label protocol |
Starting with 500ng of total RNA, Cy3 labeled cRNA was produced according to manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
For each sample, 1.65ug of Cy3 labeled cRNAs were fragmented and hybridized for 17 hours in a rotating hybridization oven.
|
| Scan protocol |
Slides were washed and then scanned with an Agilent Scanner.
|
| Description |
gene expression in WT ovariectomized saline vehicle treated uterus
|
| Data processing |
Data was obtained using the Agilent Feature Extraction software (v9.5), using the 1-color defaults for all parameters.
|
| |
|
| Submission date |
Apr 01, 2014 |
| Last update date |
Mar 25, 2015 |
| Contact name |
NIEHS Microarray Core |
| E-mail(s) |
microarray@niehs.nih.gov, liuliw@niehs.nih.gov
|
| Organization name |
NIEHS
|
| Department |
DIR
|
| Lab |
Microarray Core
|
| Street address |
111 T.W. Alexander Drive
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL4134 |
| Series (1) |
| GSE56423 |
Estradiol Induced Transcriptional Profile of EAAE DNA Binding Deficient ERa Uterus |
|
