|
| Status |
Public on Jan 06, 2015 |
| Title |
Y3-1 |
| Sample type |
RNA |
| |
|
| Source name |
regulatory T cell
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype/variation: Y3
tissue: laminai propria small and large intestine
cell type: regulatory T cells (Tregs)
|
| Treatment protocol |
T regulatory cells were FACS purified and total RNA was isolated
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using TRizol and further purified with RNAeasy Minikit (QIAGEN). RNA quantity and qulaity was confirmned by analysis with a Agilent 2100 Bioananalyzer
|
| Label |
biotin
|
| Label protocol |
10-20 ng of RNA was used in a single round of linear RNA probe amplification using Ovation Pico WTA System V2 and biotin-labeled using Encore Biotin Module (NuGEN, San Carlos, CA, USA).
|
| |
|
| Hybridization protocol |
Biotinylated cRNA was hybridized to Affymetrix Gene ST1.0 arrays
|
| Scan protocol |
Image analysis was performed using the Affymetrix Command Console Software (AGCC).
|
| Data processing |
Resulting data were normalized with the RMA method using software at GeneSifter (Seattle, WA). Group comparisons of the transformed data of 3 independent biological replicates were performed using ANOVA applying the Benjamini Hochberg correction for false positives. Data analysis was based on annotated mRNAs and setting a lower signal intensity limit of 4 units (one group must pass).
|
| |
|
| Submission date |
Apr 02, 2014 |
| Last update date |
Jan 06, 2015 |
| Contact name |
Thomas R Malek |
| E-mail(s) |
tmalek@med.miami.edu
|
| Organization name |
University of Miami
|
| Department |
Microbiology/Immunology
|
| Street address |
1600 NW 10th Avenue
|
| City |
Miami |
| State/province |
FL |
| ZIP/Postal code |
33136 |
| Country |
USA |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE56438 |
IL-2Rβ- and CD103-dependent genes in regulatory T cells in the gut mucosa |
|
