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Status |
Public on Jan 06, 2015 |
Title |
Y3-3 |
Sample type |
RNA |
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Source name |
regulatory T cell
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 genotype/variation: Y3 tissue: laminai propria small and large intestine cell type: regulatory T cells (Tregs)
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Treatment protocol |
T regulatory cells were FACS purified and total RNA was isolated
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using TRizol and further purified with RNAeasy Minikit (QIAGEN). RNA quantity and qulaity was confirmned by analysis with a Agilent 2100 Bioananalyzer
|
Label |
biotin
|
Label protocol |
10-20 ng of RNA was used in a single round of linear RNA probe amplification using Ovation Pico WTA System V2 and biotin-labeled using Encore Biotin Module (NuGEN, San Carlos, CA, USA).
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Hybridization protocol |
Biotinylated cRNA was hybridized to Affymetrix Gene ST1.0 arrays
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Scan protocol |
Image analysis was performed using the Affymetrix Command Console Software (AGCC).
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Data processing |
Resulting data were normalized with the RMA method using software at GeneSifter (Seattle, WA). Group comparisons of the transformed data of 3 independent biological replicates were performed using ANOVA applying the Benjamini Hochberg correction for false positives. Data analysis was based on annotated mRNAs and setting a lower signal intensity limit of 4 units (one group must pass).
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Submission date |
Apr 02, 2014 |
Last update date |
Jan 06, 2015 |
Contact name |
Thomas R Malek |
E-mail(s) |
tmalek@med.miami.edu
|
Organization name |
University of Miami
|
Department |
Microbiology/Immunology
|
Street address |
1600 NW 10th Avenue
|
City |
Miami |
State/province |
FL |
ZIP/Postal code |
33136 |
Country |
USA |
|
|
Platform ID |
GPL16570 |
Series (1) |
GSE56438 |
IL-2Rβ- and CD103-dependent genes in regulatory T cells in the gut mucosa |
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