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Sample GSM1361591 Query DataSets for GSM1361591
Status Public on Jan 06, 2015
Title Y3-3
Sample type RNA
 
Source name regulatory T cell
Organism Mus musculus
Characteristics strain: C57BL/6
genotype/variation: Y3
tissue: laminai propria small and large intestine
cell type: regulatory T cells (Tregs)
Treatment protocol T regulatory cells were FACS purified and total RNA was isolated
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using TRizol and further purified with RNAeasy Minikit (QIAGEN). RNA quantity and qulaity was confirmned by analysis with a Agilent 2100 Bioananalyzer
Label biotin
Label protocol 10-20 ng of RNA was used in a single round of linear RNA probe amplification using Ovation Pico WTA System V2 and biotin-labeled using Encore Biotin Module (NuGEN, San Carlos, CA, USA).
 
Hybridization protocol Biotinylated cRNA was hybridized to Affymetrix Gene ST1.0 arrays
Scan protocol Image analysis was performed using the Affymetrix Command Console Software (AGCC).
Data processing Resulting data were normalized with the RMA method using software at GeneSifter (Seattle, WA). Group comparisons of the transformed data of 3 independent biological replicates were performed using ANOVA applying the Benjamini Hochberg correction for false positives. Data analysis was based on annotated mRNAs and setting a lower signal intensity limit of 4 units (one group must pass).
 
Submission date Apr 02, 2014
Last update date Jan 06, 2015
Contact name Thomas R Malek
E-mail(s) tmalek@med.miami.edu
Organization name University of Miami
Department Microbiology/Immunology
Street address 1600 NW 10th Avenue
City Miami
State/province FL
ZIP/Postal code 33136
Country USA
 
Platform ID GPL16570
Series (1)
GSE56438 IL-2Rβ- and CD103-dependent genes in regulatory T cells in the gut mucosa

Data table header descriptions
ID_REF
VALUE Log2 RMA signal intensity

Data table
ID_REF VALUE
17210850 0.92165
17210852 1.12326
17210855 6.83449
17210869 7.54233
17210883 2.39185
17210887 5.7936
17210904 3.10636
17210912 6.91552
17210947 1.38272
17210953 2.79289
17210984 7.50684
17210994 2.91246
17210996 2.45524
17210998 1.04009
17211000 5.18998
17211004 3.12433
17211023 2.52054
17211033 1.92888
17211043 3.32811
17211066 2.1207

Total number of rows: 39137

Table truncated, full table size 645 Kbytes.




Supplementary file Size Download File type/resource
GSM1361591_Malek_ss3_A12_030413.CEL.gz 8.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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