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Sample GSM1361730 Query DataSets for GSM1361730
Status Public on Oct 01, 2014
Title 2.SIRT1 young
Sample type RNA
 
Source name SIRT1 young
Organism Mus musculus
Characteristics age: young
genotype: SIRT1F/F
cell type: microglia
genetic background: BL6 and 129/Sv
Treatment protocol Adult microglia were isolated from SIRT1F/F or LysM-Cre/SIRT1F/F mice as described with some modifications. Brains were placed in a culture dish with enzyme solution and the digested brains were triturated with a 20-ml pipette and passed through 70-μm filter. After centrifugation, cells were incubated with anti-myelin microbeads, centrifuged, resuspendend and washed. Myelin-negative fractions were collected and further processed by incubating with anti-CD11b microbeads at 4°C for 15 min. After washing, the resuspended myelin negative fraction was passed through an autoMACS Pro Separator, and then the CD11b-positive population was separated from CD11b-negative population. Cells were centrifuged, and the pellets were lysed with RNA lysis buffer (QIAGEN) for microarray or quantitative RT-PCR.
Extracted molecule total RNA
Extraction protocol RNA was extracted with standard methods
Label Biotin
Label protocol Biotinylated cRNA were prepared with Illumina kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Data processing The data were log2 transformed and normalised using quantile normalisation in R
 
Submission date Apr 02, 2014
Last update date Oct 01, 2014
Contact name Giovanni Coppola
E-mail(s) gcoppola@ucla.edu
Phone 310-794-4172
Organization name UCLA
Department Psychiatry and Neurology
Lab Neurogenetics
Street address 1524 Gonda, 695 Charles Young Drive South
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
 
Platform ID GPL6885
Series (1)
GSE56452 SIRT1 deficiency in microglia contributes to cognitive decline in aging and neurodegeneration via epigenetic regulation of IL-1beta

Data table header descriptions
ID_REF
VALUE quantile normalized

Data table
ID_REF VALUE
ILMN_1212607 7.909476937
ILMN_1212612 7.953755543
ILMN_1212619 7.624063082
ILMN_1212628 7.489457143
ILMN_1212632 7.68293468
ILMN_1212636 11.60077316
ILMN_1212637 11.23164395
ILMN_1212645 7.923708121
ILMN_1212648 9.606780229
ILMN_1212653 8.304592986
ILMN_1212672 8.985747223
ILMN_1212682 7.815104666
ILMN_1212683 7.479765723
ILMN_1212685 7.481017301
ILMN_1212692 7.727904109
ILMN_1212693 7.833695598
ILMN_1212695 12.81571755
ILMN_1212698 7.401149763
ILMN_1212702 9.213341747
ILMN_1212703 8.461177372

Total number of rows: 25697

Table truncated, full table size 624 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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