|
| Status |
Public on Mar 24, 2017 |
| Title |
Adult_CMs |
| Sample type |
RNA |
| |
|
| Source name |
Adult cardiomyocyte
|
| Organism |
Mus musculus |
| Characteristics |
strain: DBA/2J
gender: female
differentiation stage: CMs derived from mice adult heart
|
| Treatment protocol |
For cardiac differentiation, iPS cells were culture on suspension during 5 days to allow EBs formation. After 5 days, EBs were plated on gelatin coated dishes and treated with 1% of DMSO and/or 100 ng/ml of NRG-1 protein.
|
| Growth protocol |
iPS cells were maintained in serum containing media with feeder cells. Culture media consisted of 15% of serum (Hyclone) and DMEM high glucose (Gibco) supplemented with 1% P/S (Biowhittaker), 1% L-Glu (Gibco), 0.1 mM b-mercaptoehanol (Gibco), 1% non-essential amino acids (Gibco) and LIF Chemicon).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The cells were harvested with TRIzol Reagent (Invitrogen) and the RNA was extracted according to the manufacturer's instructions. As a last step of the extraction procedure, the RNA was purified with the RNeasy Mini-kit (Qiagen, Hilden, Germany).
|
| Label |
biotin
|
| Label protocol |
The sense cDNA was prepared from 300 ng of total RNA using the Ambion® WT Expression Kit. The sense strand cDNA was then fragmented and biotinylated with the Affymetrix GeneChip® WT Terminal Labeling Kit (PN 900671).
|
| |
|
| Hybridization protocol |
Labeled sense cDNA was hybridized to the Affymetrix MoGene-2_0-st chip according to the the manufacturer protocols and using GeneChip® Hybridization, Wash and Stain Kit.
|
| Scan protocol |
Genechips were scanned with the Affymetrix GeneChip® Scanner 3000.
|
| Description |
Gene expression data from adult cardiomyocytes
|
| Data processing |
Data were normalized with Bioconductor software using RMA algorithm.
|
| |
|
| Submission date |
Apr 04, 2014 |
| Last update date |
Mar 26, 2017 |
| Contact name |
Olalla Iglesias-Garcia |
| E-mail(s) |
oiglesias@alumni.unav.es
|
| Organization name |
CIMA
|
| Street address |
Pio XII 55
|
| City |
Pamplona |
| ZIP/Postal code |
31008 |
| Country |
Spain |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE56511 |
NRG-1 induces mature ventricular cardiac differentiation from iPS cells contributing to cardiac tissue repair |
|
