|
| Status |
Public on Apr 05, 2014 |
| Title |
Ovarian tumor, rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse ovarian tumor
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: mixed C57BL/6;BALB/c;129
genotype/variation: Arid1a flox/flox;Pten flox/flox
ovarian bursal injection: AdCre
tissue: ovarian tumor
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with QIAGEN RNeasy mini kit and cleaned-up with DNase I treatment in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
T50
Replicate 2 of 5.
|
| Data processing |
The data were background subtracted, quantile normalized, and log2 transformed using neqc function in limma package in R.
|
| |
|
| Submission date |
Apr 04, 2014 |
| Last update date |
Apr 05, 2014 |
| Contact name |
Ie-Ming Shih |
| Organization name |
Johns Hopkins University School of Medicine
|
| Department |
Pathology
|
| Street address |
1550 Orleans St. CRB-II, Room 305
|
| City |
Baltimore |
| State/province |
Maryland |
| ZIP/Postal code |
21231 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE56531 |
Arid1a deletion in collaboration with Pten deletion leads to ovarian tumorigenesis in mouse |
|
