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Sample GSM1364102 Query DataSets for GSM1364102
Status Public on May 31, 2014
Title Colonic epithelia wild-type mouse 2
Sample type RNA
 
Source name Mouse colonic epithelia (wild-type)
Organism Mus musculus
Characteristics tissue: Colonic epithelia
genotype/variation: wild type
age: 3 months
Treatment protocol Isolated colonic epithelial cells were stored at -80 C degree for RNA extraction.
Growth protocol Four mice from each genotype group were sacrificed at age of 3 months, and the colonic epithelial cells were isolated using 15 mM EDT buffer.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. (Invitrogen)
Label FlashTag Biotin HSR
Label protocol Total of 200 ng of total RNA were labeled using FlashTag Biotin HSR according prtotocol (Affymatrix: FlashTag™ Biotin HSR RNA Labeling Kit for Affymetrix® GeneChip® miRNA Arrays)
 
Hybridization protocol About 130 ul of Affymatrix hybridization cocktail buffer (FS450-002) were used for about 18 hours according to the FS450-002 protocl (Affymatrix).
Scan protocol Affymatrix GeneChip Scanner 3000.
Description miRNA expression from wild-type mouse colon
Data processing Expression Console™ 1.2.0.20
 
Submission date Apr 07, 2014
Last update date May 31, 2014
Contact name Wancai Yang
E-mail(s) wyang06@uic.edu
Phone 3123554154
Organization name University of Illinois at Chicago
Department Pathology
Street address 840 South Wood Street
City Chicago
State/province IL
ZIP/Postal code 60612
Country USA
 
Platform ID GPL16384
Series (1)
GSE56577 Differential expression of microRNA in Muc2-/- mouse colonic epithelial cells

Data table header descriptions
ID_REF
VALUE Log Expression Signal - RMA-DABG

Data table
ID_REF VALUE
14q0_st 0.49
14qI-1_st 0.01
14qI-1_x_st 0.01
14qI-2_st 0.79
14qI-3_x_st 0.54
14qI-4_st 0.98
14qI-4_x_st 0.85
14qI-5_st 0.79
14qI-6_st 0.06
14qI-6_x_st 0.34
14qI-7_st 0.54
14qI-8_st 0.69
14qI-8_x_st 0.38
14qI-9_x_st 0.5
14qII-10_st 0.77
14qII-10_x_st 0.83
14qII-11_st 0.49
14qII-11_x_st 0.39
14qII-12_st 0.54
14qII-12_x_st 0.21

Total number of rows: 25015

Table truncated, full table size 521 Kbytes.




Supplementary file Size Download File type/resource
GSM1364102_WY_m1-2_miRNA-3_0_.CEL.gz 904.9 Kb (ftp)(http) CEL
Processed data included within Sample table

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