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Status |
Public on Aug 05, 2015 |
Title |
Wild Type/Polyoma Mammary Tumor-1 (WT/PyMT-1) |
Sample type |
RNA |
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Source name |
Mammary tumor excised from WT/PyMT
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Organism |
Mus musculus |
Characteristics |
strain/background: C57BL/6 genotype/variation: WT/PyMT tissue: mammary tumor tissue age: 16 weeks
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Treatment protocol |
Sample was collected from mammary tumor excised from WT/PyMT and Slc6a14-/-/PyMT mice for total RNA isolation.
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Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions (Invitrogen, Life Technologies, NY).
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Label |
biotin
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Label protocol |
Biotinylated cDNAs were prepared according to the standard Ambion and Affymetrix protocol from 250 ng total RNA (The Ambion WT Expression Kit and GeneChip Terminal Labeling Kit, Affymetrix).
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Hybridization protocol |
Following labeling, cDNAs were hybridized for 16 hr at 45C on Mouse Gene 2.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
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Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
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Description |
WT/PyMT-1 Gene expression data from WT/PyMT mammary tumor tissue.
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Data processing |
Intensities of arrays have been quantile-normalized using Partek Genomic Suite (v6.6). Differential expressions were calculated using ANOVA of Partek package.
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Submission date |
Apr 08, 2014 |
Last update date |
Aug 05, 2015 |
Contact name |
Pachiappan Arjunan |
Organization name |
NUS, NIH, GRU
|
Department |
Anatomy, Unit on Retinal Vascular Neurobiology, BMB
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Lab |
VTRP, URVN, BMB
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Street address |
1120 15th ST, Laney walker Blvd,
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City |
Augusta |
State/province |
Georgia |
ZIP/Postal code |
30904 |
Country |
USA |
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Platform ID |
GPL16570 |
Series (1) |
GSE56612 |
Genetic deletion or pharmacologic blockade of the amino acid transporter Slc6a14 in mice suppresses breast cancer induced by Polyoma middle T oncogene |
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