|
| Status |
Public on Aug 05, 2015 |
| Title |
Wild Type/Polyoma Mammary Tumor-2 (WT/PyMT-2) |
| Sample type |
RNA |
| |
|
| Source name |
Mammary tumor excised from WT/PyMT
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: C57BL/6
genotype/variation: WT/PyMT
tissue: mammary tumor tissue
age: 16 weeks
|
| Treatment protocol |
Sample was collected from mammary tumor excised from WT/PyMT and Slc6a14-/-/PyMT mice for total RNA isolation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions (Invitrogen, Life Technologies, NY).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNAs were prepared according to the standard Ambion and Affymetrix protocol from 250 ng total RNA (The Ambion WT Expression Kit and GeneChip Terminal Labeling Kit, Affymetrix).
|
| |
|
| Hybridization protocol |
Following labeling, cDNAs were hybridized for 16 hr at 45C on Mouse Gene 2.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
WT/PyMT-2
Gene expression data from WT/PyMT mammary tumor tissue.
|
| Data processing |
Intensities of arrays have been quantile-normalized using Partek Genomic Suite (v6.6). Differential expressions were calculated using ANOVA of Partek package.
|
| |
|
| Submission date |
Apr 08, 2014 |
| Last update date |
Aug 05, 2015 |
| Contact name |
Pachiappan Arjunan |
| Organization name |
NUS, NIH, GRU
|
| Department |
Anatomy, Unit on Retinal Vascular Neurobiology, BMB
|
| Lab |
VTRP, URVN, BMB
|
| Street address |
1120 15th ST, Laney walker Blvd,
|
| City |
Augusta |
| State/province |
Georgia |
| ZIP/Postal code |
30904 |
| Country |
USA |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE56612 |
Genetic deletion or pharmacologic blockade of the amino acid transporter Slc6a14 in mice suppresses breast cancer induced by Polyoma middle T oncogene |
|
