|
| Status |
Public on Jan 01, 2015 |
| Title |
Cortical astrocytes 7DIV, Mecp2 308/y, biological rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
P0 mouse cortical astrocytes 7DIV, Mecp2 308/y
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl6J
cell type: Primary culture of astrocytes from cortex
genotype: Mecp2 308/y
age: P0 pups, 7 days in vitro
|
| Growth protocol |
P0 newborns from pregnant Mecp2 308/+ heterozygous mice were used for the preparation of dissociated cortical astrocyte cultures. Astrocytes were kept 7 days in vitro in DMEM supplemented with 10% FBS and 1% Pen/Strep (GIBCO)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction with the Qiagen RNeasy kit (Qiagen) as described by the manufacturer
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from total RNA (Following the GeneChip whole transcript (WT) Sense Target labelling assay kit Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, cDNA were hybridized for 16 hr at 45C on GeneChip murine Gene from Affymetrix GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GCS 3000 scanner (Affymetrix)
|
| Description |
Gene expression data
|
| Data processing |
The data were preprocessed and analyzing using Partek Genomic Suite.
|
| |
|
| Submission date |
Apr 10, 2014 |
| Last update date |
Jan 01, 2015 |
| Contact name |
Florent Dumont |
| E-mail(s) |
florent.dumont@u-psud.fr
|
| Organization name |
Université Paris-Saclay
|
| Street address |
17 avenue des sciences
|
| City |
91400 Orsay |
| State/province |
Ile de France |
| ZIP/Postal code |
91400 |
| Country |
France |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE56709 |
Astrocyte transcriptome from Mecp2-deficient mice |
|
