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Sample GSM136838 Query DataSets for GSM136838
Status Public on Feb 01, 2009
Title Female MRL/lpr Lacrimal Gland_Testosterone-2
Sample type RNA
 
Source name Lacrimal glands from testosterone treated female MRL/lpr mice ( glands pooled from 6 mice).
Organism Mus musculus
Characteristics Lacrimal glands from testosterone treated female MRL/lpr mice (glands pooled from 6 mice).
Extracted molecule total RNA
Extraction protocol RNA was purified with Trizol and RNAqueous spin columns following the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Codelink protocol from 2 microg total RNA using a CodeLink Expression Assay Reagent Kit.
 
Hybridization protocol Following fragmentation, 20 microg of cRNA were hybridized for 18 hr at 45C on a Affymetrix GeneChip Mouse Expression Array 430A using a Affymetrix hybridization oven. GeneChips were washed and stained with streptavidin-Alexa 647 and washed again using a Model 450 Fluidics station and Affymetrix GeneChip Operating Software (GCOS) .
Scan protocol GeneChips were scanned with a Affymetrix Model 7000 scanner running GCOS v1.3 software.
Description Lacrimal gland gene expression from female MRL/lpr mice treated with testosterone for 18 days.
Data processing Data were analyzed with GCOS software using the default settings. No normalization was performed.
 
Submission date Sep 19, 2006
Last update date Mar 18, 2009
Contact name David A Sullivan
E-mail(s) david.sullivan@schepens.harvard.edu
Phone (617) 912-0287
Fax (617) 912-0101
Organization name Schepens Eye Research Institute
Lab David Sullivan
Street address 20 Staniford Street
City Boston
State/province MA
ZIP/Postal code 02114
Country USA
 
Platform ID GPL8321
Series (1)
GSE5877 Female MRL/lpr and Non-Obese Diabetic (NOD) Mice Treated with Placebo or Testosterone.

Data table header descriptions
ID_REF
VALUE
ABS_CALL

Data table
ID_REF VALUE ABS_CALL
AFFX-BioB-5_at 82 P
AFFX-BioB-M_at 238 P
AFFX-BioB-3_at 178.7 P
AFFX-BioC-5_at 398.8 P
AFFX-BioC-3_at 411.6 P
AFFX-BioDn-5_at 889.3 P
AFFX-BioDn-3_at 1874.1 P
AFFX-CreX-5_at 4909.2 P
AFFX-CreX-3_at 5711.7 P
AFFX-DapX-5_at 0.5 A
AFFX-DapX-M_at 1.2 A
AFFX-DapX-3_at 3.1 A
AFFX-LysX-5_at 1.2 A
AFFX-LysX-M_at 1.6 A
AFFX-LysX-3_at 0.6 A
AFFX-PheX-5_at 0.7 A
AFFX-PheX-M_at 3.1 A
AFFX-PheX-3_at 1.4 A
AFFX-ThrX-5_at 1 A
AFFX-ThrX-M_at 0.9 A

Total number of rows: 22690

Table truncated, full table size 398 Kbytes.




Supplementary file Size Download File type/resource
GSM136838.CEL.gz 1.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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