|
| Status |
Public on Feb 01, 2009 |
| Title |
Female NOD Lacrimal Gland_Testosterone-2 |
| Sample type |
RNA |
| |
|
| Source name |
Lacrimal glands from testosterone treated female NOD mice ( glands pooled from 2-4 mice).
|
| Organism |
Mus musculus |
| Characteristics |
Lacrimal glands from testosterone treated female NOD mice (glands pooled from 2-4 mice).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was purified with Trizol and RNAqueous spin columns following the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Codelink protocol from 2 microg total RNA using a CodeLink Expression Assay Reagent Kit.
|
| |
|
| Hybridization protocol |
Following fragmentation, 20 microg of cRNA were hybridized for 18 hr at 45C on a Affymetrix GeneChip Mouse Expression Array 430A using a Affymetrix hybridization oven. GeneChips were washed and stained with streptavidin-Alexa 647 and washed again using a Model 450 Fluidics station and Affymetrix GeneChip Operating Software (GCOS) .
|
| Scan protocol |
GeneChips were scanned with a Affymetrix Model 7000 scanner running GCOS v1.3 software.
|
| Description |
Lacrimal gland gene expression from female NOD mice treated with testosterone for 21 days.
|
| Data processing |
Data were analyzed with GCOS software using the default settings. No normalization was performed.
|
| |
|
| Submission date |
Sep 19, 2006 |
| Last update date |
Mar 18, 2009 |
| Contact name |
David A Sullivan |
| E-mail(s) |
david.sullivan@schepens.harvard.edu
|
| Phone |
(617) 912-0287
|
| Fax |
(617) 912-0101
|
| Organization name |
Schepens Eye Research Institute
|
| Lab |
David Sullivan
|
| Street address |
20 Staniford Street
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
|
| Platform ID |
GPL8321 |
| Series (1) |
| GSE5877 |
Female MRL/lpr and Non-Obese Diabetic (NOD) Mice Treated with Placebo or Testosterone. |
|
