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Status |
Public on Feb 01, 2009 |
Title |
Aromatase KO Lacrimal_Male 3 |
Sample type |
RNA |
|
|
Source name |
Lacrimal glands from male aromatase knockout mice ( glands pooled from 5-8 mice)
|
Organism |
Mus musculus |
Characteristics |
Lacrimal glands from male aromatase knockout mice (glands pooled from 5-8 mice)
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was purified with Trizol and RNAqueous spin columns following the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Codelink protocol from 2 microg total RNA using a CodeLink Expression Assay Reagent Kit
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|
|
Hybridization protocol |
Following fragmentation, 20 microg of cRNA were hybridized for 18 hr at 45C on a Affymetrix GeneChip Mouse Expression Array 430A using a Affymetrix hybridization oven. GeneChips were washed and stained with streptavidin-Alexa 647 and washed again using a Model 450 Fluidics station and Affymetrix GeneChip Operating Software (GCOS) .
|
Scan protocol |
GeneChips were scanned with a Affymetrix Model 7000 scanner running GCOS v1.3 software.
|
Description |
Lacrimal gland gene expression in male aromatase knockout mice
|
Data processing |
Data were analyzed with GCOS software using the default settings. No normalization was performed.
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|
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Submission date |
Sep 19, 2006 |
Last update date |
Mar 18, 2009 |
Contact name |
David A Sullivan |
E-mail(s) |
david.sullivan@schepens.harvard.edu
|
Phone |
(617) 912-0287
|
Fax |
(617) 912-0101
|
Organization name |
Schepens Eye Research Institute
|
Lab |
David Sullivan
|
Street address |
20 Staniford Street
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02114 |
Country |
USA |
|
|
Platform ID |
GPL8321 |
Series (1) |
GSE5878 |
Lacrimal Gland Gene expression in Aromatase Knockout Mice and Wild Type Controls |
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