|
| Status |
Public on Oct 01, 2014 |
| Title |
mouse 5-5, repl1 |
| Sample type |
RNA |
| |
|
| Source name |
spleen, unsorted
|
| Organism |
Mus musculus |
| Characteristics |
leukemia type: erythroid
tissue: spleen
mouse model: Cbx7-overexpression
|
| Treatment protocol |
single cell suspensions were made from spleen. 100.000 cells were stored as pellets in -80F.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the RNeasy Mini Kit (QIAGEN)
|
| Label |
biotin
|
| Label protocol |
NuGEN Ovation PicoSL WTA System kit (# 3310-48) followed by Encore BiotinIL Module (#4210-48) was used to generate Biotin labeled ss cDNA
|
| |
|
| Hybridization protocol |
750 ng of the obtained biotinylated cDNA samples was hybridized onto the Illumina MouseRef8 v2 BeadChips according to the protocol from Illumina ”Whole-Genome Gene Expression Direct Hybridization Assay”
|
| Scan protocol |
Illumina’s Genomestudio v. 2011.1 software with default settings.
|
| Description |
102199-06
|
| Data processing |
Data was normalized using the Bioconducter library limma by control background correction, quantile normalization and log2 transformation (R version 3.0.2 neqc function).
|
| |
|
| Submission date |
Apr 15, 2014 |
| Last update date |
Oct 01, 2014 |
| Contact name |
Leonid Bystrykh |
| E-mail(s) |
l.bystrykh@umcg.nl
|
| Organization name |
University Medical Center Groningen
|
| Department |
European Research Institute for the Biology of Ageing - ERIBA
|
| Lab |
Ageing Biology and Stem Cells
|
| Street address |
A. Deusinglaan 1
|
| City |
Groningen |
| State/province |
Groningen |
| ZIP/Postal code |
9713 AV |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE56820 |
Leukemia dynamics and heterogeneity traced by barcoding in a pre-clinical Cbx7 mouse model |
|
