|
| Status |
Public on Feb 19, 2015 |
| Title |
MDA-MB-231 UF010, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Human breast cancer cell line MDA-MB-231 treated with UF010
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Cultured breast cancer cells
cell line: MDA-MB-231
|
| Treatment protocol |
DMSO (1 µl) was added to cell culture with 1 ml of medium. Similarly, 1 µl of 1 mM UF010 was added to the final concentration of 1 µM. Cells were cultured for 24 hours after treatment.
|
| Growth protocol |
MDA-MB-231 cells were seeded in a well in a 6-well plate and cultured in complete DMEM medium for 24 hours
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA with the RNeasy mini kit was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200 ng total RNA
|
| |
|
| Hybridization protocol |
Sense strand DNA was fragmented, biotinylated, and hybridized with rotation at 45°C for 16 hr to the GeneChip® Human Transcriptome Array 2.0.
|
| Scan protocol |
The arrays were washed and stained with streptavidin-phycoerythrin (SAPE) on an Affymetrix Fluidics Station 450, and scanned on a GeneChip® 7G Scanner (Affymetrix, Inc).
|
| Description |
Gene expression data from MDA-MB-231 cells treated with UF010 at 1 µM for 24 hours
|
| Data processing |
We used the Affymetrix® Expression Console™ Software (Version 1.3) to generate .txt files for each RNA hybridization.
|
| |
|
| Submission date |
Apr 15, 2014 |
| Last update date |
Feb 19, 2015 |
| Contact name |
Daiqing Liao |
| E-mail(s) |
dliao@ufl.edu
|
| Phone |
3522738188
|
| Organization name |
University of Florida
|
| Department |
Anatomy and Cell Biology
|
| Street address |
1333 Center Drive
|
| City |
Gainesville |
| State/province |
FLORIDA |
| ZIP/Postal code |
32610 |
| Country |
USA |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE56823 |
Expression data from MDA-MB-231 breast cancer cell lines exposed to DMSO control and UF010 |
|
