|
| Status |
Public on May 01, 2015 |
| Title |
211At_spleen_1.7kBq_1h_#2 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse spleen, 1.7 kBq, 1h
|
| Organism |
Mus musculus |
| Characteristics |
strain: Balb/c
gender: female
tissue: spleen
injected activity: 1.7 kBq
time point: 1h
nuclide: 211-astatine
|
| Treatment protocol |
Excised organs were flash-frozen in liquid nitrogen and stored at -80°C until RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with RNeasy Lipid Tissue Mini Kit according to the manufacturer’s instructions. Quality control was performed with Agilent Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
Illumina®Totalprep-96RNA Amplification kit 4397949 RevC, (Modification of Amplification kit v 2.2)
|
| |
|
| Hybridization protocol |
WGGEX_Direct_Hybridization_Assay_EUC_11322187_A
|
| Scan protocol |
Standard Illumina iScan N240 scanning protocol with scan setting file = Direct Hyb
|
| Data processing |
The data were normalized using quantile normalization with BASE
|
| |
|
| Submission date |
Apr 17, 2014 |
| Last update date |
May 01, 2015 |
| Contact name |
Britta Langen |
| E-mail(s) |
britta.langen@gu.se
|
| Organization name |
Sahlgrenska Academy at University of Gothenburg
|
| Department |
Department of Radiation Physics
|
| Street address |
Gula stråket 2B
|
| City |
Gothenburg |
| ZIP/Postal code |
SE-413 45 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE56894 |
Transcriptional response in normal tissues in mice after 211At administration - dependency of absorbed dose, dose-rate and time |
|
