|
| Status |
Public on Apr 16, 2015 |
| Title |
IP Pomc-Cre:RiboTag mouse_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Immunoprecipitated polysome RNA, Pomc-Cre mice crossed to RiboTag mouse
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Pomc-derived lineage
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the RNeasy micro kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
RNA was amplified using the Ovation SL WTA system (Nugen) prior to biotinilation according to the EncoreIL biotinilation kit (Nugen).
|
| |
|
| Hybridization protocol |
Biotinilated cDNA was hybridized at 48ºC to MouseRef-8 v2 expression beadchips (Illumina) for 16h before washing and analyzing according to manufacturer’s directions.
|
| Scan protocol |
Signal was detected using a Beadarray Reader (Illumina)
|
| Description |
9254035037_H
replicate 2
|
| Data processing |
Data was analyzed for differential expression using the GenomeStudio data analysis software (Illumina). Average normalization, the Illumina custom error model and multiple testing corrections using Benjamini and Hochberg False discovery rate were applied to the analysis. Only transcripts with a differential score >13 were considered.
|
| |
|
| Submission date |
Apr 18, 2014 |
| Last update date |
Apr 16, 2015 |
| Contact name |
Elisenda Sanz |
| E-mail(s) |
elisenda.sanz@uab.cat
|
| Organization name |
Universitat Autonoma de Barcelona
|
| Department |
Institut de Neurociencies
|
| Street address |
M1/113 Facultat de Medicina
|
| City |
Bellaterra |
| State/province |
Barcelona |
| ZIP/Postal code |
08193 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE56917 |
Identification of the hypothalamic neuronal populations that derive from Pomc-expressing precursors |
|
