|
| Status |
Public on Nov 01, 2014 |
| Title |
4B wt 9756 |
| Sample type |
RNA |
| |
|
| Source name |
4B wt 9756
|
| Organism |
Mus musculus |
| Characteristics |
strain: B6C3F1/J
genotype/variation: wt
treatment: 4B
tissue: gastrocnemius skeletal muscle
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA from gastrocnemius skeletal muscle was extracted using RNAeasy mini kit (Qiagen) with DNase I treatment to eliminate genomic DNA contamination
|
| Label |
biotin
|
| Label protocol |
Standard Illumina biotin-labeling protocol
|
| |
|
| Hybridization protocol |
Samples were hybridized onto the Illumina Sentrix BeadChip Array MouseRef-8 v2 for 18 hours at 58°C (protocol available at http://www.illumina.com/products/mouseref-8_expression_beadchip_kits_v2.ilmn).
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
4B_wt_9756
|
| Data processing |
The data were normalized using quantile normalization with IlluminaGUI in R
|
| |
|
| Submission date |
Apr 22, 2014 |
| Last update date |
Nov 01, 2014 |
| Contact name |
Giovanni Coppola |
| E-mail(s) |
gcoppola@ucla.edu
|
| Phone |
310-794-4172
|
| Organization name |
UCLA
|
| Department |
Psychiatry and Neurology
|
| Lab |
Neurogenetics
|
| Street address |
1524 Gonda, 695 Charles Young Drive South
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE56963 |
The effect of HDAC inhibitor, 4b, on skeletal muscle gene expression |
|
