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Sample GSM1372673 Query DataSets for GSM1372673
Status Public on May 02, 2014
Title Ezh2_Delete_4day_3
Sample type RNA
 
Source name Differentiated NSCs
Organism Mus musculus
Characteristics age: P7-P9
tissue: SVZ
Treatment protocol Ezh2 was acutely deleted by the addition of 4-hydroxytamoxifen (4OHT) to SVZ NSC cultures generated from UBC-CreERT2;Ezh2F/F from P3-4
Growth protocol SVZ NSCs were passaged 5 times in proliferation medium(DMEM/F12/N2 – Invitrogen, 5% FCS - Hyclone, 20 ng ml-1 EGF, 20 ng ml-1 bFGF, 35 μg ml-1 bovine pituitary extract – Peprotech) before switching to differentiation media (same as proliferation media except with 2% FCS and no EGF or bFGF) for the specified time.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated by Trizol (Life Technologies), purified using the RNeasy Plus Mini Kit (Qiagen) and quantified using the NanoDrop spectrophotometer. Samples were checked for quality using an Agilent TapeStation
Label biotin
Label protocol Biotinylated cRNA was generated from total RNA using an Ambion Illumina RNA amplification kit (Ambion)
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicate 3
Data processing The data were normalised using quantile normalisation with IlluminaGUI in R
 
Submission date Apr 23, 2014
Last update date May 02, 2014
Contact name William Hwang
Organization name UCSF
Department Neurosurgery
Lab Lim
Street address Lim Lab - Box 0525, 35 Medical Center Way
City San Francisco
State/province CA
ZIP/Postal code 94143
Country USA
 
Platform ID GPL6885
Series (1)
GSE56988 Timecourse analysis of Ezh2-dependent gene expression by SVZ NSCs during differentiation

Data table header descriptions
ID_REF
VALUE quantile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1248788 141.8815448 0.5827067
ILMN_2707227 148.45825 0.245614
ILMN_2896528 4679.119042 0
ILMN_2721178 1332.618833 0
ILMN_1227723 486.8778292 0
ILMN_3033922 2618.138708 0
ILMN_3092673 9709.211833 0
ILMN_2730714 5459.455167 0
ILMN_3162224 2389.281542 0
ILMN_2816356 161.4168 0.02130326
ILMN_2808939 1099.930408 0
ILMN_2634564 900.7497667 0
ILMN_1216623 1061.671279 0
ILMN_1215157 1326.563292 0
ILMN_1228777 137.4864286 0.8070176
ILMN_2737647 157.4059042 0.05263158
ILMN_1216425 148.8844806 0.2255639
ILMN_2734484 613.16225 0
ILMN_2652961 141.202484 0.6165413
ILMN_2952292 306.7269125 0

Total number of rows: 25697

Table truncated, full table size 786 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Raw data are available on Series record

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