|
| Status |
Public on Oct 07, 2014 |
| Title |
Nek7 cells, cortex, TRAP RNA, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
TRAP RNA
|
| Organism |
Mus musculus |
| Characteristics |
genetic background: C57BL/6 / FVB
expression: Nek7
cell type: cortical interneuron
line: Nek7 MN733
area: Cortex
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRAP affinity purification of ribosomes from BAC transgenic mice (see accompanying manuscript), followed by Trizol-LS extraction, DNAse treatement, and RNeasy Minelute Cleanup.
|
| Label |
biotin
|
| Label protocol |
Affymetrix two cycle amplification kit, starting with 20 ng of total RNA.
|
| |
|
| Hybridization protocol |
Standard Affymetric protocol for mouse 430 2.0 platform.
|
| Scan protocol |
Standard Affymetric protocol for mouse 430 2.0 platform.
|
| Description |
TRAP RNA
Nek7_ctx_ip2
|
| Data processing |
GCRMA normlalization within biological replicate groups was performed with R/Bioconductor
|
| |
|
| Submission date |
Apr 23, 2014 |
| Last update date |
Oct 07, 2014 |
| Contact name |
Miho Nakajima |
| Organization name |
The Rockefeller University
|
| Lab |
Heintz
|
| Street address |
1230 York Ave.
|
| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL18376 |
| Series (1) |
| GSE56996 |
Comparative analysis of different cortical interneuron groups. |
|
