|
Status |
Public on Oct 07, 2014 |
Title |
Htr3a cells, cortex, TRAP RNA, biological rep1 |
Sample type |
RNA |
|
|
Source name |
TRAP RNA
|
Organism |
Mus musculus |
Characteristics |
genetic background: C57BL/6 / FVB expression: Htr3a cell type: cortical interneuron line: Htr3a GM433 area: Cortex
|
Extracted molecule |
total RNA |
Extraction protocol |
TRAP affinity purification of ribosomes from BAC transgenic mice (see accompanying manuscript), followed by Trizol-LS extraction, DNAse treatement, and RNeasy Minelute Cleanup.
|
Label |
biotin
|
Label protocol |
Affymetrix two cycle amplification kit, starting with 20 ng of total RNA.
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|
|
Hybridization protocol |
Standard Affymetric protocol for mouse 430 2.0 platform.
|
Scan protocol |
Standard Affymetric protocol for mouse 430 2.0 platform.
|
Description |
TRAP RNA Htr3a_ctx_ip1
|
Data processing |
GCRMA normlalization within biological replicate groups was performed with R/Bioconductor
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|
|
Submission date |
Apr 23, 2014 |
Last update date |
Oct 07, 2014 |
Contact name |
Miho Nakajima |
Organization name |
The Rockefeller University
|
Lab |
Heintz
|
Street address |
1230 York Ave.
|
City |
New York |
State/province |
New York |
ZIP/Postal code |
10065 |
Country |
USA |
|
|
Platform ID |
GPL18376 |
Series (1) |
GSE56996 |
Comparative analysis of different cortical interneuron groups. |
|