|
| Status |
Public on Jun 05, 2009 |
| Title |
ACE1, 24hour palmitate exposure, biol rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
Human hepatocyte HepG2 cell line, stably transfected with pBudaceAB
|
| Organism |
Homo sapiens |
| Characteristics |
HepG2 cell line stably transfected with pBudaceAB
Experiments done before pasage 10
|
| Treatment protocol |
Cells were plated in 10cm plate at 70% confluency. Cells were washed with PBS and then given DMEM augmented with 300uM palmitate for 24 hours, at which point total RNA was harvested.
|
| Growth protocol |
pBudaceAB transfected HepG2 cell line grown in DMEM augmented with 300uM palmitate.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
15ug of biotin-tagged cRNA was fragmented to an average strand length of 100 bases (range 35-200 bases) following prescribed protocols.
|
| |
|
| Hybridization protocol |
10 ug of fragmented target cRNA was hybridized at 45 degrees C with rotation for 16 hours (Affymetrix GeneChip Hybridization Oven 640) to probe sets present on an Affymetrix HG_U133A 2 array. The GeneChip arrays were washed and then stained (SAPE, streptavidin-phycoerythrin) on an Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Description |
Gene expression data from wild type HepG2 cell line after 24 hour 300uM palmiate exposure.
|
| Data processing |
The results were quantified and analyzed using GCOS 1.2 software using default values (Scaling, Target Signal Intensity = 500; Normalization, All Probe Sets; Parameters, all set at default values).
|
| |
|
| Submission date |
Sep 22, 2006 |
| Last update date |
Oct 26, 2008 |
| Contact name |
Jason Thaddeus Dean |
| E-mail(s) |
jtdean@ucla.edu
|
| Phone |
310-206-1642
|
| Organization name |
UCLA
|
| Department |
Chemical and Biomolecular Engineering
|
| Lab |
Liao
|
| Street address |
420 Westwood Plaza
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE5903 |
A synthetic gene-metabolic circuit preferentially increased fatty acid metabolism in human hepatocytes |
|
