|
| Status |
Public on Apr 25, 2014 |
| Title |
Normal |
| Sample type |
RNA |
| |
|
| Source name |
Normal
|
| Organism |
Mus musculus |
| Characteristics |
strain: Balb/c
cell type: Primary macrophage
stress: normal control (not Leishmania-infected)
time: control
|
| Treatment protocol |
Infected with an MOI 10 of Leishmania donovani
|
| Growth protocol |
Macrophages grown in 60 mm plates
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA prepared using Trizol according to manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
MicroRNA expression data
SAMPLE 1
|
| Data processing |
The data were analyzed with Expression Console and Gene springGX 12.0 using Affymetrix default analysis settings and RMA as normalization method used. The probes of intensities less than the 20.0 percentile in the raw data were excluded.
|
| |
|
| Submission date |
Apr 24, 2014 |
| Last update date |
Apr 25, 2014 |
| Contact name |
Syamal Roy |
| E-mail(s) |
sroy@iicb.res.in
|
| Phone |
919432962207
|
| Organization name |
Indian Institute of Chemical Biology
|
| Department |
Infectious Disease and Immunology
|
| Street address |
4, Raja SC Mullick Road
|
| City |
Kolkata |
| State/province |
West Bengal |
| ZIP/Postal code |
700032 |
| Country |
India |
| |
|
| Platform ID |
GPL16384 |
| Series (1) |
| GSE57042 |
microRNA expression profile in Leishmania donovani infected mouse macrophage |
|
